Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A highly automated 96-well solid phase extraction and liquid chromatography/tandem mass spectrometry method for the determination of fentanyl in human plasma
Autore:
Shou, WZ; Jiang, XY; Beato, BD; Weng, ND;
Indirizzi:
Covance Labs Inc, Bioanalyt Chem, Madison, WI 53704 USA Covance Labs Inc Madison WI USA 53704 oanalyt Chem, Madison, WI 53704 USA Covance Bioanalyt Chem, Indianapolis, IN 46214 USA Covance Bioanalyt ChemIndianapolis IN USA 46214 dianapolis, IN 46214 USA
Titolo Testata:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
fascicolo: 7, volume: 15, anno: 2001,
pagine: 466 - 476
SICI:
0951-4198(2001)15:7<466:AHA9SP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LC-MS-MS; HUMAN URINE; QUANTITATIVE-DETERMINATION; THROUGHPUT BIOANALYSIS; PROTEIN PRECIPITATION; ULTRAVIOLET DETECTION; SAMPLE PREPARATION; PLATE FORMAT; ALFENTANIL; LC/MS/MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Weng, ND Covance Labs Inc, Bioanalyt Chem, 3301 Kinsman Blvd, Madison, WI 53704 USA Covance Labs Inc 3301 Kinsman Blvd Madison WI USA 53704 53704 USA
Citazione:
W.Z. Shou et al., "A highly automated 96-well solid phase extraction and liquid chromatography/tandem mass spectrometry method for the determination of fentanyl in human plasma", RAP C MASS, 15(7), 2001, pp. 466-476

Abstract

A high-throughput bioanalytical method based on automated sample transfer,automated solid phase extraction, and fast liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis, has been developed for the determination of the analgesic fentanyl in human plasma. Samples were transferred into96-well plates using an automated sample handling system. Automated solid phase extraction (SPE) was carried out using a 96-channel programmable liquid-handling workstation using a mixed-mode sorbent. The extracted samples were then dried down, reconstituted and injected onto a silica column using an aqueous/organic mobile phase with tandem mass spectrometric detection. The method has been validated over the concentration range 0.05-100 ng/mL fentanyl in human plasma, based on a 0.25-mL sample size. The assay is sensitive, specific and robust. More than 2000 samples have been analyzed using this method. The automation of the sample preparation steps not only increased the analysis throughput, but also facilitated the transfer of the methodbetween different bioanalytical laboratories of the same organization. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:32:43