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Titolo:
Toward rules for 1 : 1 polyamide : DNA recognition
Autore:
Urbach, AR; Dervan, PB;
Indirizzi:
CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA CALTECH Pasadena CAUSA 91125 iv Chem & Chem Engn, Pasadena, CA 91125 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 8, volume: 98, anno: 2001,
pagine: 4343 - 4348
SICI:
0027-8424(20010410)98:8<4343:TRF1:1>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE-SPECIFIC RECOGNITION; DOUBLE-HELICAL DNA; MINOR-GROOVE; BASE-PAIRS; IMIDAZOLE POLYAMIDES; B-DNA; MOTIF; PYRROLE; OLIGOPEPTIDES; DISTAMYCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Dervan, PB CALTECH, Div Chem & Chem Engn, 164-30, Pasadena, CA 91125 USA CALTECH 164-30 Pasadena CA USA 91125 30, Pasadena, CA 91125 USA
Citazione:
A.R. Urbach e P.B. Dervan, "Toward rules for 1 : 1 polyamide : DNA recognition", P NAS US, 98(8), 2001, pp. 4343-4348

Abstract

Polyamides composed of four amino acids, imidazole (Im), pyrrole (Py), hydroxypyrrole (Hp), and beta -alanine (beta) are synthetic ligands that form highly stable complexes in the minor groove of DNA. Although specific pairing rules within the 2:1 motif can be used to distinguish the four Watson Crick base pairs, a comparable recognition code for 1:1 polyamide:DNA complexes had not been described. To set a quantitative baseline for the field, the sequence specificities of Im, Py, Hp, and beta for the four Watson Crick base pairs were determined for two polyamides, Im-beta -ImPy-beta -Im-beta -ImPy-beta -DP (1, for Im, Py, and beta) and Im-beta -ImHp-beta -Im-beta -ImPy-beta -Dp (2, for Hp), in a 1:1 complex within the DNA sequence context 5'-AAA-GAGAAGAG-3'. Im residues do not distinguish C,C from A,T but bind all four base pairs with high affinity. Py and beta residues exhibit greater than or equal to 10-fold preference for A,T over G,C base pairs. The Hp residue displays a unique preference for a single A.T base pair with an energetic penalty.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:37:54