Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The balance of concurrent aggregation and deaggregation processes in platelets is linked to differential occupancy of ADP receptor subtypes
Autore:
Maayani, S; Tagliente, TM; Schwarz, T; Martinelli, G; Martinez, R; Shore-Lesserson, L;
Indirizzi:
Mt Sinai Sch Med, Dept Anesthesiol, Basic Res Lab, New York, NY USA Mt Sinai Sch Med New York NY USA hesiol, Basic Res Lab, New York, NY USA
Titolo Testata:
PLATELETS
fascicolo: 2, volume: 12, anno: 2001,
pagine: 83 - 93
SICI:
0953-7104(200103)12:2<83:TBOCAA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC FLOW VARIATIONS; ISOLATED RABBIT AORTA; MICROPLATE READER; BLOOD-PLATELETS; MOLECULAR-BASIS; SHAPE CHANGE; ACTIVATION; KINETICS; CLOPIDOGREL; AGONISTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Maayani, S CUNY, Mt Sinai Med Ctr, Dept Anesthesiol, Box 1010, New York, NY 10029 USA CUNY Box 1010 New York NY USA 10029 010, New York, NY 10029 USA
Citazione:
S. Maayani et al., "The balance of concurrent aggregation and deaggregation processes in platelets is linked to differential occupancy of ADP receptor subtypes", PLATELETS, 12(2), 2001, pp. 83-93

Abstract

Deaggregation, the partial reversal of the initial aggregation of platelets is observed following low, but not higher, micromolar ADP concentrations. This study tested the hypothesis that deaggregation results from a balancebetween concurrent, opposing, aggregation and deaggregation processes which are ADP (adenosine 5'-diphosphate) receptor occupancy-dependent, Aggregation of human platelet-rich plasma (PRP) prepared in r-hirudin was assayed in a 96-well plate reader over 20 min by measurement of the optical density (OD) at 580 nm, Aggregation and the time to reach peak aggregation were directly proportional to ADP receptor occupancy. The magnitude and time courseof the response to ADP were comparable to those previously reported with standard aggregometry, The rate constant of platelet deaggregation, as assessed by a four-compartment kinetic model, was inversely proportional to agonist concentration. The ratio of the rate constants of aggregation and deaggregation was receptor occupancy-dependent and directly proportional to aggregation. Consequently, platelet aggregation was proportional, and deaggregation inversely proportional, to ADP receptor occupancy, We propose that theresponse of PRP to ADP and to 2-MeS-ADP (2-methylthioadenosine-diphosphate), in vitro, consists of at least two active, concurrent processes, aggregation and deaggregation, Incremental occupancy of the P-2T ADP receptor subtype attenuates deaggregation and governs the balance between these two processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:05:26