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Titolo:
Platelet CD40 ligand (CD40L) - subcellular localization, regulation of expression, and inhibition by clopidogrel
Autore:
Hermann, A; Rauch, BH; Braun, M; Schror, K; Weber, AA;
Indirizzi:
Univ Dusseldorf, Inst Pharmakol & Klin Pharmakol, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 ol, D-4000 Dusseldorf, Germany
Titolo Testata:
PLATELETS
fascicolo: 2, volume: 12, anno: 2001,
pagine: 74 - 82
SICI:
0953-7104(200103)12:2<74:PCL(-S>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; HUMAN BLOOD-PLATELETS; ENDOTHELIAL-CELLS; PROTEIN; ATHEROSCLEROSIS; AGGREGATION; ACTIVATION; MEMBRANES; CD154;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Schror, K Moorenstr 5, D-40225 Dusseldorf, Germany Moorenstr 5 Dusseldorf Germany D-40225 225 Dusseldorf, Germany
Citazione:
A. Hermann et al., "Platelet CD40 ligand (CD40L) - subcellular localization, regulation of expression, and inhibition by clopidogrel", PLATELETS, 12(2), 2001, pp. 74-82

Abstract

This study compares the subcellular localization and the regulation of expression of the platelet activation markers CD62P and CD63 with CD30 ligand (CD40L) on the surface of washed human platelets, CD40L was expressed upon stimulation with a wide range of platelet activators, However, quantitativeflow cytometry demonstrated that, as compared with CD62P and CD63, CD40L expression was low. Upon stimulation with thrombin receptor-activating peptide (TRAP-6), all activation markers were expressed, In contrast, upon stimulation with low concentrations of collagen (1-3 mug/ml), CD40L, but not thegranule proteins (CD62P, CD63), mere expressed. Using immunofluorescence microscopy, a cytoplasmic staining was observed for CD40L, and cytoplasmic localization of CD40L was verified by Western blotting of subcellular platelet fractions, The staining of CD40L was different from that of filamentous actin and only little association of CD40L with platelet cytoskeleton was found, Surface expression of CD40L was dependent on internal Ca2+ stores andprotein kinase C, white the mitogen-activated protein kinases (ERK, p38) or tyrosine kinases were not involved. ADP (30 muM)-induced CD40L expressionwas not inhibited by aspirin. In contrast, clopidogrel treatment completely abolished ADP-induced expression of CD40L. Finally, the expression level of CD40L was shown to be upregulated by phorbol myristate acetate (PMA) in the promegakaryocytic cell line MEG-01.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:10:13