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Titolo:
Hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines with different radiothermosensitivity in vitro
Autore:
Islam, MS; Mitsuhashi, N; Akimoto, T; Sakurai, H; Hasegawa, M; Ishikawa, H; Niibe, H;
Indirizzi:
Gunma Univ, Sch Med, Dept Radiol & Radiat Oncol, Gunma 3718511, Japan Gunma Univ Gunma Japan 3718511 diol & Radiat Oncol, Gunma 3718511, Japan
Titolo Testata:
ONCOLOGY REPORTS
fascicolo: 3, volume: 8, anno: 2001,
pagine: 501 - 507
SICI:
1021-335X(200105/06)8:3<501:HAITRY>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; RADIOSENSITIVITIES IN-VITRO; P53 GENE; THYMOCYTE APOPTOSIS; DNA FRAGMENTATION; DEATH; EXPRESSION; RADIATION; CANCER; COMBINATION;
Keywords:
hyperthermia; apoptosis; p53; heat shock protein; rat yolk sac tumor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Islam, MS Gunma Univ, Sch Med, Dept Radiol & Radiat Oncol, 3-39-22 Showa Machi, Gunma 3718511, Japan Gunma Univ 3-39-22 Showa Machi Gunma Japan 3718511 18511, Japan
Citazione:
M.S. Islam et al., "Hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines with different radiothermosensitivity in vitro", ONCOL REP, 8(3), 2001, pp. 501-507

Abstract

We investigated cell susceptibility to hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines (RYSTs) and attempted to correlate this with the known potentially relevant molecular determinants of apoptosis, p53 protein status, Bcl-2 family of proteins and heat shock proteins (Hsp). Parent cell line, NMT-1 (carrying wild-type p53 gene) was radiosensitive but thermoresistant compared to the variant cell line, NMT-1R (mutated type p53), which was isolated from NMT-1 by repeated radiation exposure. Induction of apoptosis by hyperthermia at 43 degreesC was morphologically detected in both RYSTs using hematoxylin and eosin, and TUNEL staining and additionallyconfirmed by DNA ladder formation (the cleavage of DNA into oligonucleosomal fragments). Western blot analysis showed an increase in expression of p53, p21(WAF1/CIP1), Hsp70 proteins in both cell lines after heat-shock at 43degreesC for 30 min. Hsp90 expression increased in NMT-1 but was not affected by heating in NMT-1R cells, whereas hyperthermia exerted no effect on the endogenous expression of Pax. Bcl-2 protein could not be detected in either RYST. These results suggest that hyperthermia induced apoptosis in bothNMT-1 and NMT-1R and apoptosis in RYSTs may be independent of p53-dependent signaling pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:29:01