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Titolo:
Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front
Autore:
Paweletz, CP; Charboneau, L; Bichsel, VE; Simone, NL; Chen, T; Gillespie, JW; Emmert-Buck, MR; Roth, MJ; Petricoin, EF; Liotta, LA;
Indirizzi:
NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA US FDA, Ctr Biol Evaluat & Res, Tissue Prote Unit, Bethesda, MD 20892 USA US FDA Bethesda MD USA 20892 s, Tissue Prote Unit, Bethesda, MD 20892 USA NCI, Canc Prevent Studies Branch, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 ent Studies Branch, NIH, Bethesda, MD 20892 USA NCI, Pathogenet Unit, Pathol Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 t Unit, Pathol Lab, NIH, Bethesda, MD 20892 USA NCI, Canc Genome Anat Project, Off Director, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 ject, Off Director, NIH, Bethesda, MD 20892 USA Georgetown Univ, Dept Chem, Washington, DC 20057 USA Georgetown Univ Washington DC USA 20057 pt Chem, Washington, DC 20057 USA
Titolo Testata:
ONCOGENE
fascicolo: 16, volume: 20, anno: 2001,
pagine: 1981 - 1989
SICI:
0950-9232(20010412)20:16<1981:RPPMWC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTATIC INTRAEPITHELIAL NEOPLASIA; GLYCOGEN-SYNTHASE KINASE-3; NERVE GROWTH-FACTOR; PROTEOMIC ANALYSIS; GENE-EXPRESSION; APOPTOSIS; AKT; IMMUNOASSAY; SPECIFICITY; ARRAYS;
Keywords:
laser capture microdissection; protein microarrays; apoptosis; Akt; mitogen activated protein kinase; tumor progression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Liotta, LA NCI, Pathol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA NCI Bldg 10 Bethesda MD USA 20892 dg 10, Bethesda, MD 20892 USA
Citazione:
C.P. Paweletz et al., "Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front", ONCOGENE, 20(16), 2001, pp. 1981-1989

Abstract

Protein arrays are described for screening of molecular markers and pathway targets in patient matched human tissue during disease progression. In contrast to previous protein arrays that immobilize the probe, our reverse phase protein array immobilizes the whole repertoire of patient proteins thatrepresent the state of individual tissue cell populations undergoing disease transitions. A high degree of sensitivity, precision and linearity was achieved, making it possible to quantify the phosphorylated status of signalproteins in human tissue cell subpopulations, Using this novel protein microarray we have longitudinally analysed the state of pro-survival checkpoint proteins at the microscopic transition stage from patient matched histologically normal prostate epithelium to prostate intraepithelial neoplasia (PIN) and then to invasive prostate cancer. Cancer progression was associatedwith increased phosphorylation of Akt (P < 0.04), suppression of apoptosispathways (P < 0.03), as well as decreased phosphorylation of ERK (P < 0.01), At the transition from histologically normal epithelium to PIN we observed a statistically significant surge in phosphorylated Akt (P < 0.03) and aconcomitant suppression of downstream apoptosis pathways which proceeds the transition into invasive carcinoma.

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Documento generato il 27/09/20 alle ore 04:54:41