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Titolo:
DNA methyltransferases of the cyanobacterium Anabaena PCC 7120
Autore:
Matveyev, AV; Young, KT; Meng, A; Elhai, J;
Indirizzi:
Univ Richmond, Dept Biol, Richmond, VA 23173 USA Univ Richmond Richmond VA USA 23173 nd, Dept Biol, Richmond, VA 23173 USA
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 7, volume: 29, anno: 2001,
pagine: 1491 - 1506
SICI:
0305-1048(20010401)29:7<1491:DMOTCA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP-STRAIN PCC-7120; SEQUENCE-SPECIFIC ENDONUCLEASES; RESTRICTION-MODIFICATION SYSTEM; HORIZONTAL GENE-TRANSFER; ESCHERICHIA-COLI; POLYPHYLETIC ORIGIN; GENOME; METHYLASE; DAM; RECOGNITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Elhai, J Univ Richmond, Dept Biol, Richmond, VA 23173 USA Univ Richmond Richmond VA USA 23173 Biol, Richmond, VA 23173 USA
Citazione:
A.V. Matveyev et al., "DNA methyltransferases of the cyanobacterium Anabaena PCC 7120", NUCL ACID R, 29(7), 2001, pp. 1491-1506

Abstract

From the characterization of enzyme activities and the analysis of genomicsequences, the complement of DNA methyltransferases (MTases) possessed by the cyanobacterium Anabaena PCC 7120 has been deduced. Anabaena has nine DNA MTases, Four are associated with Type II restriction enzymes (Aval, Avail, AvaIII and the newly recognized inactive AvalV), and five are not, Of thelatter, four may be classified as solitary MTases, those whose function lies outside of a restriction/modification system. The group is defined here based on biochemical and genetic characteristics. The four solitary MTases,DmtA/M.AvaVI, DmtB/M.AvaVII, DmtC/M,AvaVIII and DmtD/M,AvalX, methylate atGATC, GGCC, CGATCG and rCCGGy, respectively. DmtB methylates cytosines at the N4 position, but its sequence is more similar to NG-adenine MTases thanto cytosine-specific enzymes, indicating that it may have evolved from theformer. The solitary MTases, appear to be of ancient origin within cyanobacteria, while the restriction MTases appear to have arrived by recent horizontal transfer as did five now inactive Type I restriction systems, One Mtase, M.AvaV, cannot reliably be classified as either a solitary or restriction MTase, It is structurally unusual and along with a few proteins of prokaryotic and eukaryotic origin defines a structural class of MTases distinct from all previously described.

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Documento generato il 10/04/20 alle ore 16:06:39