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Titolo:
Imaging stochastic spatial variability of active channel clusters during excitation of single neurons
Autore:
Savtchenko, LP; Gogan, P; Korogod, SM; Tyc-Dumont, S;
Indirizzi:
Unite Neurocybernet Cellulaire, F-13009 Marseille, France Unite Neurocybernet Cellulaire Marseille France F-13009 arseille, France Dniepropetrovsk State Univ, Res Lab Biophys & Bioelect, Natl Acad Sci Ukraine, Int Ctr Mol Physiol,Dniepropetrovsk Div, UA-49050 Dniepropetrovsk, Ukraine Dniepropetrovsk State Univ Dniepropetrovsk Ukraine UA-49050 vsk, Ukraine
Titolo Testata:
NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 39, anno: 2001,
pagine: 431 - 446
SICI:
0168-0102(200104)39:4<431:ISSVOA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
VOLTAGE-SENSITIVE DYE; NODOSE GANGLION-CELLS; ION CHANNELS; ACTION-POTENTIALS; PYRAMIDAL NEURONS; DENDRITES; MEMBRANE; FLUORESCENCE; PROPAGATION; SOMA;
Keywords:
cell imaging; active ion channels; channel clusters; spatial patterns of fluorescence; noise; stochastic model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Tyc-Dumont, S Unite Neurocybernet Cellulaire, 280 Bd St Marguerite, F-13009 Marseille, France Unite Neurocybernet Cellulaire 280 Bd St Marguerite Marseille France F-13009
Citazione:
L.P. Savtchenko et al., "Imaging stochastic spatial variability of active channel clusters during excitation of single neurons", NEUROSCI RE, 39(4), 2001, pp. 431-446

Abstract

Topographical maps of membrane voltages were obtained during action potentials by imaging, at 1 mum resolution, live dissociated neurons stained withthe voltage sensitive dye RH237. We demonstrate with a theoretical approach that the spatial patterns in the images result from the distribution of net positive charges condensed in the inner sites of the membrane where clusters of open ionic channels are located. We observed that, in our biological images, this spatial distribution of open channels varies randomly from trial to trial while the action potentials recorded by the microelectrode display similar amplitudes and time-courses. The random differences in size and intensity of the spatial patterns in the images are best evidenced when the time of observation coincides with the duration of single action potentials. This spatial variability is explained by the fact that only part of the channel population generates an action potential and that different channels open in turn in different trials due to their stochastic operation. Such spatial flicker modifies the direction of lateral current along the neuronal membrane and may have important consequences on the intrinsic processing capabilities of the neuron. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:23:41