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Titolo:
Differential expression of glutamate and GABA-A receptor subunit mRNA in cortical dysplasia
Autore:
Crino, PB; Duhaime, AC; Baltuch, G; White, R;
Indirizzi:
Univ Penn, Dept Neurol, Sch Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 , Sch Med, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Neurosurg, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Neurosurg, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Penn Epilepsy Ctr, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 lepsy Ctr, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA
Titolo Testata:
NEUROLOGY
fascicolo: 7, volume: 56, anno: 2001,
pagine: 906 - 913
SICI:
0028-3878(20010410)56:7<906:DEOGAG>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURONAL MIGRATION DISORDERS; DEVELOPING RAT-BRAIN; IN-VITRO RECORDINGS; MESSENGER-RNAS; NEOCORTICAL STRUCTURES; ALZHEIMERS-DISEASE; NMDA-RECEPTORS; EPILEPSY; MODEL; HYPEREXCITABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Crino, PB Univ Penn, Dept Neurol, Sch Med, 3 W Gates Bldg,3400 Spruce St, Philadelphia, PA 19104 USA Univ Penn 3 W Gates Bldg,3400 Spruce St Philadelphia PA USA 19104
Citazione:
P.B. Crino et al., "Differential expression of glutamate and GABA-A receptor subunit mRNA in cortical dysplasia", NEUROLOGY, 56(7), 2001, pp. 906-913

Abstract

Objective: Focal cortical dysplasia is characterized by disorganized cortical lamination, dysplastic and heterotopic neurons, and an association withepilepsy. The contribution that dysplastic and heterotopic neurons make toepileptogenesis in focal cortical dysplasia is unknown and the phenotype of these cells may be distinct. The authors hypothesized that the expressionof genes encoding glutamatergic (glutamate [GluR] and N-methyl-D-aspartateNMDA receptors [NR]) and gamma -aminobutyric acid A receptor (GABA(A)R) subunits is distinct in dysplastic and heterotopic neurons and that changes in receptor gene expression could be defined in a cell-specific pattern. Methods: Single immunohistochemically labeled dysplastic and heterotopic neurons were microdissected from human focal cortical dysplasia specimens obtained during epilepsy surgery. Pyramidal neurons were microdissected from postmortem control cortex and from temporal cortex without dysplasia resected during temporal lobectomy. Poly (A) messenger RNA (mRNA) from single neuronswas amplified, radiolabeled, and used to probe complementary DNA (cDNA) arrays containing GluR(1-6), NR1A,1B, NR2A-D, and GABA(A)R alpha (1-6), and -R beta (1-3) subunit cDNAs. The relative hybridization intensities of each mRNA-cDNA hybrid were quantified by phosphorimaging. Results: GluR, NR, andGABA(A)R subunit mRNA expression did not differ between control neurons and nondysplastic epilepsy specimens. Expression of GluR(4), NR2B, and NR2C subunit mRNA was increased, and NR2A and GABA(A)R beta (1) subunit mRNA was decreased in dysplastic compared with pyramidal and heterotopic neurons. Incontrast, GABA(A)R alpha (1), -R alpha (2), and -R beta (2) as well as GluR(1) mRNA levels were reduced in both dysplastic and heterotopic neurons. Conclusions: Differential expression of GluR, NR, and GABA(A)R mRNA in dysplastic and heterotopic neurons demonstrates cell specific gene transcriptionchanges in focal cortical dysplasia. These results suggest that dysplasticand heterotopic neurons may be pharmacologically distinct and make differential contributions epileptogenesis in focal cortical dysplasia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:39:09