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Titolo:
Infection of human airway epithelia with H1N1, H2N2, and H3N2 influenza A virus strains
Autore:
Slepushkin, VA; Staber, PD; Wang, GS; McCray, PB; Davidson, BL;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Physiol & Biophys, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ysiol & Biophys, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Neurol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Neurol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Program Gene Therapy, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 am Gene Therapy, Iowa City, IA 52242 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 395 - 402
SICI:
1525-0016(200103)3:3<395:IOHAEW>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; RECEPTOR SPECIFICITY; APICAL SURFACE; CELLS; FUSION; HEMAGGLUTININ; SELECTION; BINDING; ENTRY; DETERMINANTS;
Keywords:
HA; gene therapy; pseudotype;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Davidson, BL Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Iowa City, IA 52242 USA
Citazione:
V.A. Slepushkin et al., "Infection of human airway epithelia with H1N1, H2N2, and H3N2 influenza A virus strains", MOL THER, 3(3), 2001, pp. 395-402

Abstract

Three subtypes of influenza A virus cause human disease: H1N1, H2N2, and H3N2. Although all result in respiratory illness, little is known about how these subtypes infect differentiated airway epithelia. Therefore, we assayed A/PR/8/34 (H1N1), A/Japan/305/57 (H2N2), and X31 (H3N2) influenza virus strains for binding and infection on fully differentiated primary cultures of airway epithelia isolated from human bronchus, grown on semiporous filters at an air-liquid interface. In this model system, viral infectivity was highest when virus was applied to the apical versus the basolateral surface;japan was most infectious, followed by PR8. The X31 strain showed very lowlevels of infectivity. Confocal microscopy and fluorescence-resonance energy transfer studies indicated that Japan virus could enter and fuse with cellular membranes, while infection with X31 virions was greatly inhibited. japan virus could also productively infect human trachea explant tissues. These data show that influenza viruses with SA alpha2,3Gal binding specificity, like Japan, productively infect differentiated human airway epithelia from the apical surface. These data are important to consider in the development of pseudotyped recombinant viral vectors for gene transfer to human airway epithelia for gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 22:13:53