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Titolo:
Transduction of liver cells by lentiviral vectors: Analysis in living animals by fluorescence imaging
Autore:
Pfeifer, A; Kessler, T; Yang, M; Baranov, E; Kootstra, N; Cheresh, DA; Hoffman, RM; Verma, IM;
Indirizzi:
Salk Inst Biol Studies, Genet Lab, La Jolla, CA 92037 USA Salk Inst Biol Studies La Jolla CA USA 92037 Lab, La Jolla, CA 92037 USA Scripps Clin & Res Inst, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 nst, La Jolla, CA 92037 USA AntiCanc Inc, San Diego, CA 92111 USA AntiCanc Inc San Diego CA USA 92111AntiCanc Inc, San Diego, CA 92111 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 319 - 322
SICI:
1525-0016(200103)3:3<319:TOLCBL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSFER; IN-VIVO; EXPRESSION; DELIVERY; PROTEIN; HIV-1; MICE;
Keywords:
lentiviral vectors; gene transfer; fluorescence imaging; GFP; liver; hepatocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Verma, IM Salk Inst Biol Studies, Genet Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA Salk Inst Biol Studies 10010 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
A. Pfeifer et al., "Transduction of liver cells by lentiviral vectors: Analysis in living animals by fluorescence imaging", MOL THER, 3(3), 2001, pp. 319-322

Abstract

Viral vectors based on lentiviruses, such as the human immunodeficiency virus, are able to transduce a broad spectrum of nondividing cells in vivo. This ability of lentiviral vectors makes them an attractive vehicle for genetransfer into the liver. In order to determine the requirements for efficient lentiviral gene transfer, we used a fluorescence imaging system, which allows the detection of cells and tissues that express fluorescent reportergenes (e.g., green fluorescence protein) in the living animal. We show that the latest generation of lentiviral vectors efficiently transduces the murine liver. Further analysis demonstrated that neither cell-cycle activation nor division of liver cells is a prerequisite for lentiviral gene transfer in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/02/20 alle ore 21:00:56