Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression of 11 beta-hydroxysteroid dehydrogenase isozymes and corticosteroid hormone receptors in primary cultures of human trophoblast and placental bed biopsies
Autore:
Driver, PM; Kilby, MD; Bujalska, I; Walker, EA; Hewison, M; Stewart, PM;
Indirizzi:
Univ Birmingham, Queen Elizabeth Hosp, Div Med Sci, Birmingham B15 2TH, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TH W Midlands, England Univ Birmingham, Queen Elizabeth Hosp, Div Reprod & Child Hlth, BirminghamB15 2TH, W Midlands, England Univ Birmingham Birmingham W Midlands EnglandB15 2TH W Midlands, England
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 4, volume: 7, anno: 2001,
pagine: 357 - 363
SICI:
1360-9947(200104)7:4<357:EO1BDI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; MINERALOCORTICOID RECEPTOR; FETAL MEMBRANES; HUMAN-PREGNANCY; TYPE-2; INVASION; TISSUES; CYTOTROPHOBLASTS; LOCALIZATION; SPECIFICITY;
Keywords:
11 beta-hydroxysteroid dehydrogenase; glucocorticoid receptor; mineralocorticoid receptor; placenta; trophoblast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Driver, PM Univ Birmingham, Queen Elizabeth Hosp, Div Med Sci, Birmingham B15 2TH, W Midlands, England Univ Birmingham Birmingham W Midlands EnglandB15 2TH England
Citazione:
P.M. Driver et al., "Expression of 11 beta-hydroxysteroid dehydrogenase isozymes and corticosteroid hormone receptors in primary cultures of human trophoblast and placental bed biopsies", MOL HUM REP, 7(4), 2001, pp. 357-363

Abstract

Interconversion of active and inactive glucocorticoids, e.g. cortisol (F) and cortisone (E) is catalysed by 11 beta -hydroxysteroid dehydrogenase (11beta -HSD) which exists as two isoforms. We have used human placental bed biopsies and an in-vitro cytotrophoblast cell culture system to examine theexpression and activity of the 11 beta -HSD isoforms along with that of the glucocorticoid and mineralocorticoid receptors (GR and MR). Immunohistochemistry localized 11 beta -HSD1 to decidualized stromal cells and 11 beta -HSD2 to villous cytotrophoblast, syncytiotrophoblasts and trophoblast cellsinvading the placental bed and maternal vasculature. In primary cultures of human cytotrophoblast, 11 beta -HSD2, GR and MR mRNA were expressed. Low levels of 11 beta -HSD1 mRNA were noted in these cultured cells, but could be explained on the basis of contaminating, vimentin-positive decidual stromal cells (less than or equal to5%). Enzyme activity studies confirmed the presence of a high-affinity, NAD dependent dehydrogenase activity (K-m 137 nmol/l and V-max 128 pmol E/h/mg protein), indicative of the 11 beta -HSD2 isoform. No reductase activity was observed. The presence of functional MR and GR was determined using Scatchard analyses of dexamethasone and aldosterone binding (MR K-d 1.4 nmol/l B-max 3.0; GR K-d 6.6 nmol/l B-max 16.2 fmol/ng protein). The expression of 11 beta -HSD1 in maternal decidua and 11P-HSD2 in adjacent trophoblast suggests an important role for glucocorticoidsin determining trophoblast invasion. The presence of the MR within trophoblast indicates that some of the effects of cortisol could be MR- rather than GR-mediated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:24:51