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Titolo:
Contribution of cryofixation and freeze-substitution to analytical microscopy: A study of Tritrichomonas foetus hydrogenosomes
Autore:
Ribeiro, KC; Benchimol, M; Farina, M;
Indirizzi:
Univ Santa Ursula, BR-22231010 Rio De Janeiro, Brazil Univ Santa Ursula Rio De Janeiro Brazil BR-22231010 BCDe Janeiro, Brazil Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 e Janeiro, Brazil Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biol Celular & Tecidual, Campos, RJ, Brazil Univ Estadual Norte Fluminense Campos RJ Brazil dual, Campos, RJ, Brazil Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Anat, Lab Biomineralizacao, Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil o, Rio De Janeiro, Brazil
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 1, volume: 53, anno: 2001,
pagine: 87 - 92
SICI:
1059-910X(20010401)53:1<87:COCAFT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENERGY-LOSS SPECTROSCOPY; ELECTRON-MICROSCOPY; CALCIUM; MEMBRANES; CELLS; FETUS;
Keywords:
hydrogenosomes; Tritrichomonas foetus; high-pressure freezing; freeze-substitution; X-ray microanalysis; electron spectroscopic imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Benchimol, M Univ Santa Ursula, Rua Jornalista Orlando Dantas,36 Botafogo,BR-22231010 Rio De Janeiro, Brazil Univ Santa Ursula Rua Jornalista Orlando Dantas,36 Botafogo Rio De Janeiro Brazil BR-22231010 BC
Citazione:
K.C. Ribeiro et al., "Contribution of cryofixation and freeze-substitution to analytical microscopy: A study of Tritrichomonas foetus hydrogenosomes", MICROSC RES, 53(1), 2001, pp. 87-92

Abstract

The hydrogenosome, an organelle that produces molecular hydrogen and ATP from the oxidation of pyruvate or malate under anaerobic conditions, presents some characteristics common to mitochondria. It is found in several trichomonad species, protists living in oxygen-poor environments, as well as certain free-living ciliates, rumen ciliates, and some fungi. We performed a comparative microanalytical study (energy dispersive X-ray analysis and electron spectroscopic imaging) of different fixation methods for electron microscopy analyzing hydrogenosomes of the bovine parasite Tritrichomonas foetus. The study included the elemental composition and the mapping of calcium,phosphorus, and oxygen. A preparation of T. foetus cells, based on cryoimmobilization by high-pressure freezing and freeze-substitution, was comparedto a second preparation based on chemical fixation followed by dehydrationand routine processing. The ultrastructural preservation achieved by the cryotechnique was far superior to the chemical fixation, since it allowed the successful cryoimmobilization of intracellular ion contents. The detection of several cations (Al, Mg, Co, Ca, Fe) by X-ray microanalysis inside theperipheral vesicle of the hydrogenosome was only possible in cryofixed cells. The presence of aluminum and cobalt ion in the hydrogenosomal vesicle was established for the first time. Electron-spectroscopic images of calciumshowed that this element, in addition to the vesicle compartment, is present in the hydrogenosome's membrane in varying concentrations, which might reflect changes in the physiology of this organelle. Microsc. Res. Tech. 53:87-92, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 12:37:49