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Titolo:
Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296
Autore:
Adams, MM; Allison, GE; Verma, NK;
Indirizzi:
Australian Natl Univ, Fac Sci, Sch Life Sci, Div Biochem & Mol Biol, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 rra, ACT 0200, Australia
Titolo Testata:
MICROBIOLOGY-UK
, volume: 147, anno: 2001,
parte:, 4
pagine: 851 - 860
SICI:
1350-0872(200104)147:<851:TIOAMG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSYL TRANSFERASE GENE; ESCHERICHIA-COLI K-12; O-ANTIGEN; MOLECULAR CHARACTERIZATION; SEROTYPE CONVERSION; BACTERIOPHAGE-SFV; VACCINE STRAIN; ENCODING GENE; CLONING; LIPOPOLYSACCHARIDE;
Keywords:
bacteriophage; serotype conversion; lipopolysaccharide; glucosyltransferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Verma, NK Australian Natl Univ, Fac Sci, Sch Life Sci, Div Biochem & Mol Biol, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 200, Australia
Citazione:
M.M. Adams et al., "Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296", MICROBIO-UK, 147, 2001, pp. 851-860

Abstract

The genes encoding type IV O antigen glucosylation were characterized fromboth Escherichia coli and Shigella flexneri. The putative O antigen modification genes from E. coli, o120 o306 o443, were PCR-amplified and introduced into S. flexneri serotype Y strain SFL124. Immunogold labelling and phagesensitivity indicated the presence of both serotype Y and serotype 4a O antigens on the cell surface of the resulting recombinant SFL124 strains, suggesting that only partial serotype conversion was conferred by the E. coli genes. The type IV O antigen modification genes were then isolated and characterized from S. flexneri serotype 4a strain NCTC 8296. A 3.8 kb chromosomal fragment conferred complete conversion to serotype 4a when introduced into SFL124. Sequence analysis of the fragment revealed the presence of threegenes, gtrA(IV) gtrB(IV) gtrIV(Sf). DNAs homologous to bacteriophage int and attP were located upstream of gtrA(IV), suggesting that this region of the NCTC 8296 genome may have originated from a bacteriophage; however, a serotype-converting phage could not be induced from this strain nor from of her strains used in this study. Comparison of the GtrIV(Sf) and GtrIV(Ec) (o443) proteins revealed that they are 41% identical and 63% similar, which is the highest degree of similarity reported among the S. flexneri O antigenglucosyltransferases.

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Documento generato il 25/09/20 alle ore 10:17:58