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Titolo:
Identification of the mycobacterial DNA-binding protein 1 region which suppresses transcription in vitro
Autore:
Furugen, M; Matsumoto, S; Matsuo, T; Matsumoto, M; Yamada, T;
Indirizzi:
Nagasaki Univ, Sch Dent, Dept Oral Bacteriol, Nagasaki 8528588, Japan Nagasaki Univ Nagasaki Japan 8528588 Bacteriol, Nagasaki 8528588, Japan Nagasaki Univ, Sch Dent, Dept Oral Surg 1, Nagasaki 8528588, Japan Nagasaki Univ Nagasaki Japan 8528588 ral Surg 1, Nagasaki 8528588, Japan Otsuka Pharmaceut Co Ltd, Tokushima 7710192, Japan Otsuka Pharmaceut Co Ltd Tokushima Japan 7710192 okushima 7710192, Japan
Titolo Testata:
MICROBIAL PATHOGENESIS
fascicolo: 3, volume: 30, anno: 2001,
pagine: 129 - 138
SICI:
0882-4010(200103)30:3<129:IOTMDP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTONE-LIKE PROTEINS; ESCHERICHIA-COLI; GENE-EXPRESSION; CHROMATIN STRUCTURE; REPLICATION; H1; AFFINITY; BACTERIA; TOPOLOGY; DISEASE;
Keywords:
Mycobacterium; DNA-binding protein; transcription; slow growth; mapping;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Furugen, M Nagasaki Univ, Sch Dent, Dept Oral Bacteriol, Sakamoto 1-7-1, Nagasaki 8528588, Japan Nagasaki Univ Sakamoto 1-7-1 Nagasaki Japan 8528588588, Japan
Citazione:
M. Furugen et al., "Identification of the mycobacterial DNA-binding protein 1 region which suppresses transcription in vitro", MICROB PATH, 30(3), 2001, pp. 129-138

Abstract

We recently identified a novel DNA-binding protein from Mycobacterium bovis bacillus Calmette-Guerin (BCG), termed mycobacterial DNA-binding protein 1 (MDP1). MDP1 inhibited the in vitro syntheses of DNA, RNA and protein, and reduced growth rates of bacteria transformed with MDP1 genes. In this study, we examined the DNA binding regions of MDP1 by using a set of syntheticpeptides. One dominant region was determined on peptide 4 composed of amino acids, at positions 31-50. The peptide 4 inhibited syntheses of both DNA and RNA in vitro. The critical amino acids residues for these functions were analysed utilizing synthetic peptides substituted with Ala. This domain was perfectly conserved in MDP1 homologues in mycobacteria, but not observedin other known DNA binding proteins. These results indicate mycobacteria possess a unique nuclear protein, which might be involved in growth regulation of these organisms. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:00:42