Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Genetic targeting of an adenovirus vector via replacement of the fiber protein with the phage T4 fibritin
Autore:
Krasnykh, V; Belousova, N; Korokhov, N; Mikheeva, G; Curiel, DT;
Indirizzi:
Univ Alabama, Div Human Gene Therapy, Dept Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 , Dept Med, Birmingham, AL 35294 USA Univ Alabama, Div Human Gene Therapy, Dept Pathol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Pathol, Birmingham, AL 35294 USA Univ Alabama, Div Human Gene Therapy, Dept Surg, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Dept Surg, Birmingham, AL 35294 USA VectorLog Inc, Birmingham, AL 35294 USA VectorLog Inc Birmingham AL USA 35294 orLog Inc, Birmingham, AL 35294 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 9, volume: 75, anno: 2001,
pagine: 4176 - 4183
SICI:
0022-538X(200105)75:9<4176:GTOAAV>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYPE-5 FIBER; RECEPTOR; BACTERIOPHAGE-T4; GENERATION; DELIVERY; TROPISM; CELLS; COXSACKIEVIRUS; THERAPY; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Curiel, DT 1824 6th Ave S,WTI 620, Birmingham, AL 35294 USA 1824 6th Ave S,WTI 620 Birmingham AL USA 35294 m, AL 35294 USA
Citazione:
V. Krasnykh et al., "Genetic targeting of an adenovirus vector via replacement of the fiber protein with the phage T4 fibritin", J VIROLOGY, 75(9), 2001, pp. 4176-4183

Abstract

The utility of adenovirus (Ad) vectors for gene therapy is restricted by their inability to selectively transduce disease-affected tissues. This limitation may be overcome by the derivation of vectors capable of interacting with receptors specifically expressed in the target tissue. Previous attempts to alter Ad tropism by genetic modification of the Ad fiber have had limited success due to structural conflicts between the fiber and the targeting ligand. Here we present a strategy to derive an Ad vector with enhanced targeting potential by a radical replacement of the fiber protein in the Ad capsid with a chimeric molecule containing a heterologous trimerization motif and a receptor-binding ligand. Our approach, which capitalized upon the overall structural similarity between the human Ad type 5 (Ad5) fiber and bacteriophage T4 fibritin proteins, has resulted in the generation of a genetically modified Ad5 incorporating chimeric fiber-fibritin proteins targeted to artificial receptor molecules. Gene transfer studies employing this novel viral vector have demonstrated its capacity to efficiently deliver a transgene payload to the target tells in a receptor-specific manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:27:59