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Titolo:
Replacement of the V3 region of gp120 with SDF-1 preserves the infectivityof T-cell line-tropic human immunodeficiency virus type 1
Autore:
Yonezawa, A; Hori, T; Takaori-Kondo, A; Morita, R; Uchiyama, T;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Hematol Oncol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 tol Oncol, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 9, volume: 75, anno: 2001,
pagine: 4258 - 4267
SICI:
0022-538X(200105)75:9<4258:ROTVRO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; ENVELOPE GLYCOPROTEIN; CHEMOKINE RECEPTORS; RETROVIRAL VECTORS; HIGH-TITER; CONFORMATIONAL-CHANGES; FUSION COFACTOR; HIV-1 INFECTION; GENE-TRANSFER; CD4(+) CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Hori, T Kyoto Univ, Grad Sch Med, Dept Hematol Oncol, Sakyo Ku, 54 ShogoinKawaramachi, Kyoto 6068507, Japan Kyoto Univ 54 Shogoin Kawaramachi KyotoJapan 6068507 8507, Japan
Citazione:
A. Yonezawa et al., "Replacement of the V3 region of gp120 with SDF-1 preserves the infectivityof T-cell line-tropic human immunodeficiency virus type 1", J VIROLOGY, 75(9), 2001, pp. 4258-4267

Abstract

Interaction between the human immunodeficiency virus type I (HIV-1) envelope and the relevant chemokine receptors is crucial for subsequent membrane fusion and viral entry, Although the V3 region of gp120 is known to determine the cell tropism as well as the coreceptor usage, the significance of the binding of the V3 region to the chemokine receptor has not been fully understood, To address this issue, we adopted the pseudotyped virus infection assay in which the V3 region of the T-cell line-tropic (T-tropic) NL4-3 envelope was replaced with a portion of stromal cell derived factor 1 (SDF-1),the ligand of CXCR4. The V3 region of the NL4-3 envelope expression vectorwas replaced with three different stretches of SDF-1 cDNA, Expression of each chimeric envelope protein was confirmed by immunoprecipitation and Western blotting. Luciferase reporter viruses were prepared by cotransfection of the pNL4-3.Luc.E-R- vector and each chimeric envelope expression vector, and the infection assay was then carried out, We showed that pseudotyped viruses with one of the chimeric envelopes, NL4-3/SDF1-51, could infect U87.CD4.CXCR4 but not U87.CD4 or U87.CXCR4 cells and that this infection was inhibited by the ligand of CXCR4, SDF-1 beta by anti-human SDF-1 antibody, or by an anti-CD4 antibody, Leu3a, in a dose-dependent manner, Furthermore, chimeric NL-4-3/SDF1-51 gp120 significantly inhibited binding of labeled SDF-1 to CXCR4. It was suggested that replacement of the V3 region of the NL4-3envelope with SDF-1 preserved the CD4-dependent infectivity of T-tropic HIV-1, These results indicate that binding between the V3 region and the relevant coreceptor is important for viral entry, whether its amino acid sequence is indigenous to the virus or not.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:32:17