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Titolo:
Autologous bone marrow implantation induced angiogenesis and improved deteriorated exercise capacity in a rat ischemic hindlimb model
Autore:
Ikenaga, S; Hamano, K; Nishida, M; Kobayashi, T; Li, TS; Kobayashi, S; Matsuzaki, M; Zempo, N; Esato, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Surg 1, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Physiol 1, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Internal Med 2, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan
Titolo Testata:
JOURNAL OF SURGICAL RESEARCH
fascicolo: 2, volume: 96, anno: 2001,
pagine: 277 - 283
SICI:
0022-4804(200104)96:2<277:ABMIIA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL PROGENITOR CELLS; INTRAMUSCULAR GENE-TRANSFER; FIBROBLAST GROWTH-FACTOR; PERIPHERAL ARTERIAL INSUFFICIENCY; COLLATERAL VESSEL DEVELOPMENT; MUSCLE BLOOD-FLOW; THERAPEUTIC ANGIOGENESIS; POSTNATAL NEOVASCULARIZATION; INFARCTED MYOCARDIUM; LIMB ISCHEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ikenaga, S Yamaguchi Univ, Sch Med, Dept Surg 1, 1-1-1 Minami-Kogushi, Ube, Yamaguchi7558505, Japan Yamaguchi Univ 1-1-1 Minami-Kogushi Ube YamaguchiJapan 7558505
Citazione:
S. Ikenaga et al., "Autologous bone marrow implantation induced angiogenesis and improved deteriorated exercise capacity in a rat ischemic hindlimb model", J SURG RES, 96(2), 2001, pp. 277-283

Abstract

Background. Bone marrow possesses endothelial progenitor cells that secrete several growth factors and can contribute to the formation of new capillaries. In the present study, we investigated the extent of angiogenesis induced by implantation of autologous bone marrow cells (BMCs) in a rat ischemic hindlimb model and studied whether the increased collateral vessels can improve deteriorated physical function. Materials and methods. Ischemic hindlimb was created by ligation of the femoral artery and its branches in Dark Agouti (DA) rats. BMCs (1 x 10(7)) were injected percutaneously at six points into the gastrocnemius muscle. To assess angiogenesis, histologic evaluation and microangiography were performed at 2 weeks postligation. Severity of the ischemic insult was evaluated by measuring blood flow in the adductor and gastrocnemius muscles using nonradioactive colored microspheres and by determining the femoral arteriovenous oxygen difference (AVDO(2)) at 2 weeks postligation. Running time on a motor-driven treadmill was used to represent exercise capacity. Results. The histologic evaluation and microangiogram showed that the implanted BMCs induce angiogenesis. Blood flow to the adductor muscle on the treated side in the bone marrow cell implantation (BMI) group was significantly restored to 77.3 +/- 19.3% of that of the normally perfused limb in comparison to that in control groups (P < 0.05). AVDO(2) in the BMI group significantly decreased when compared with AVDO(2) in control groups. Rats in the BMI group ran approximately 1.5 times longer than rats in control groups at 2 and 4 weeks postligation (P < 0.01). Conclusions. Implantation of autologous BMCs induced angiogenesis and improved deteriorated exercise capacity in our rat ischemic hindlimb model. (C)2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 02:20:17