Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Photodissociation of the CO complex of horseradish peroxidase studied by laser-induced optoacoustic spectroscopy
Autore:
Feis, A; Angeloni, L;
Indirizzi:
Univ Florence, Dept Chem, I-50121 Florence, Italy Univ Florence FlorenceItaly I-50121 Dept Chem, I-50121 Florence, Italy
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 13, volume: 105, anno: 2001,
pagine: 2638 - 2643
SICI:
1520-6106(20010405)105:13<2638:POTCCO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESONANCE RAMAN-SPECTROSCOPY; SPERM WHALE CARBOXYMYOGLOBIN; CARBON-MONOXIDE; PHOTOACOUSTIC CALORIMETRY; GEMINATE RECOMBINATION; SALT BRIDGE; MYOGLOBIN; PHOTOLYSIS; HEMOGLOBIN; RESOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Feis, A Univ Florence, Dept Chem, Via Gino Capponi 9, I-50121 Florence, Italy Univ Florence Via Gino Capponi 9 Florence Italy I-50121 ce, Italy
Citazione:
A. Feis e L. Angeloni, "Photodissociation of the CO complex of horseradish peroxidase studied by laser-induced optoacoustic spectroscopy", J PHYS CH B, 105(13), 2001, pp. 2638-2643

Abstract

Laser-induced optoacoustic spectroscopy (LIOAS) was applied to the study of the photolysis of the CO-ligated heme protein horseradish peroxidase isoenzyme C (HRP). Laser photolysis produced structural volume changes faster than 50 ns. The photoreaction volume and enthalpy changes were determined bymeans of temperature-dependent measurements in the range 6-23 degreesC. The volume change (+29.6 mL/mol) can be mainly attributed to the displacementof CO to the bulk solvent. The enthalpy change is mainly related to the Fe-C bond energy with little contribution from the protein matrix. The results are interpreted in terms of the structural properties of HRP, which has adirect exit channel from the heme to the solvent, and compared to related studies on the CO complexes with myoglobin and hemoglobin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:29:55