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Titolo:
Dental unit waterline contamination and its possible implications during periodontal surgery
Autore:
Putnins, EE; Di Giovanni, D; Bhullar, AS;
Indirizzi:
Univ British Columbia, Fac Dent, Dept Oral Biol & Med Sci, Lab PeriodontalBiol, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada
Titolo Testata:
JOURNAL OF PERIODONTOLOGY
fascicolo: 3, volume: 72, anno: 2001,
pagine: 393 - 400
SICI:
0022-3492(200103)72:3<393:DUWCAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE; SOLUBLE CD14; MICROBIAL-CONTAMINATION; FIBROBLASTS; BIOFILMS; CELLS; EXPRESSION; ENDOTOXIN; BACTERIA; DISEASE;
Keywords:
dental equipment; infection control; water pollution/prevention and control; risk factors; wound healing; biofilms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Putnins, EE Univ British Columbia, Fac Dent, Dept Oral Biol & Med Sci, LabPeriodontalBiol, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 1Z3, Canada
Citazione:
E.E. Putnins et al., "Dental unit waterline contamination and its possible implications during periodontal surgery", J PERIODONT, 72(3), 2001, pp. 393-400

Abstract

Background: Dental unit waterline contamination has become a concern to clinical dentistry. This concern arises from the fact that bacteria sloughed from established biofilms in dental unit waterlines increase heterotrophic bacteria counts in water exiting these units. Methods: Scanning microscopy and bacterial viability staining were used toexamine the sessile and planktonic biofilm present in dental unit waterlines and water samples, respectively. In addition, the limulus amebocyte assay was used to measure the lipopolysaccharide (LPS) levels in water samples. Results: All dental unit waterlines were coated with a well-established biofilm made up of filamentous and bacillus-like microorganisms. Water samples collected from these dental units contained high numbers of individual bacteria and bacterial aggregates. A viability staining technique identified significantly more bacteria in water than could be cultured, and 64% of thetotal bacterial population stained as nonvital. Since the bacterial load (viable and nonviable) was high, we examined the LPS in dental unit water samples. The mean LPS levels in water collected from high-speed and air/waterlines in use were 480 and 1,008 endotoxin units (EU)/ml. This was significantly higher than the mean level of 66 EU/ml found in water samples collected from adjacent clinic sinks. The LPS level at the start of the day (2,560EU/ml) was reduced by 70% with 1 minute of flushing (800 EU/ml). Flushing times of 5 and 10 minutes were not able to reduce LPS levels to zero. Conclusion: The presence of high heterotrophic bacterial counts, sloughingbiofilm, and high LPS levels are discussed in relation to patient risk andperiodontal wound healing biology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 02:47:07