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Titolo:
Activin type II receptors in embryonic dorsal root ganglion neurons of thechicken
Autore:
Kos, K; Fine, L; Coulombe, JN;
Indirizzi:
Uniformed Serv Univ Hlth Sci, Dept Anat & Cell Biol, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA
Titolo Testata:
JOURNAL OF NEUROBIOLOGY
fascicolo: 2, volume: 47, anno: 2001,
pagine: 93 - 108
SICI:
0022-3034(200105)47:2<93:ATIRIE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; VASOACTIVE INTESTINAL POLYPEPTIDE; GENE-RELATED PEPTIDE; MESSENGER RIBONUCLEIC-ACIDS; PRIMARY AFFERENT NEURONS; SENSORY NEURONS; SYMPATHETIC NEURONS; DIFFERENTIATION FACTOR; CILIARY GANGLION; CALCITONIN-GENE;
Keywords:
activin; activin receptor; dorsal root ganglion; vasoactive intestinal polypeptide; calcitonin gene related peptide; chicken embryo; in situ hybridization; immunocytochemistry; cell culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Coulombe, JN NINDS, Neural Dev Sect, NIH, 36 Convent Dr,MSC 4064, Bethesda, MD 20892 USA NINDS 36 Convent Dr,MSC 4064 Bethesda MD USA 20892 20892 USA
Citazione:
K. Kos et al., "Activin type II receptors in embryonic dorsal root ganglion neurons of thechicken", J NEUROBIOL, 47(2), 2001, pp. 93-108

Abstract

Activin induces neuropeptide expression in thicken ciliary ganglion neurons. To determine if activin might also influence neuropeptide expression in developing sensory neurons, we examined,whether type II activin receptors are expressed during embryonic development of the chicken dorsal root ganglia (DRG), and also examined the effects of activin on neuropeptide! expression in cultured DRG neurons, Using reverse transcription polymerase chain reaction (rtPCR), we detected mRNAs for both the activin receptors type IIA (ActRIIA) and type IIB (ActRIIB) in DRG from embryonic day 7 through posthatch day 1, With bl situ hybridization, we found that morphologically identifiable neurons express mRNAs for both ActRIIA and ActRIIB, With developmental age, a subset of neurons that hybridizes more intensely with riboprobes to these receptor mRNAs becomes evident. A similar pattern of expression is observed with immunocytochemical staining using antisera against activin type II receptors. To examine whether embryonic DRG cells respond to activin we treated dissociated cultures of DRG with activin A and assessed the expression of vasoactive intestinal peptide (VIP) and calcitonin gene related peptide (CGRP) mRNAs using semiquantitative rtPCR, Activin treatment resultsin an increase in VIP mRNA, but does not affect CGRP mRNA levels. These observations indicate that neurons in the embryonic chicken DRG can respond to activin and suggest that activin has the potential to play a role in the development and function of DRG sensory neurons. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:41:35