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Titolo:
Angiotensin II receptor antagonist blocks the expression of connexin43 induced by cyclical mechanical stretch in cultured neonatal rat cardiac myocytes
Autore:
Shyu, KG; Chen, CC; Wang, BW; Kuan, PL;
Indirizzi:
Shin Kong Wu Ho Su Mem Hosp, Dept Internal Med, Div Cardiol, Taipei 111, Taiwan Shin Kong Wu Ho Su Mem Hosp Taipei Taiwan 111 ardiol, Taipei 111, Taiwan
Titolo Testata:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
fascicolo: 4, volume: 33, anno: 2001,
pagine: 691 - 698
SICI:
0022-2828(200104)33:4<691:AIRABT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAP JUNCTION CHANNELS; MULTIPLE CONNEXINS; MOLECULAR CHARACTERIZATION; VENTRICULAR MYOCYTES; INDUCED HYPERTROPHY; GENE-EXPRESSION; INVITRO; HEART; FIBROBLASTS; CELLS;
Keywords:
stretch; cardiomyocytes; connexin43; gap junctions; angiotensin II; antagonist;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Shyu, KG Shin Kong Wu Ho Su Mem Hosp, Dept Internal Med, Div Cardiol, 95 Wen Chang Rd, Taipei 111, Taiwan Shin Kong Wu Ho Su Mem Hosp 95 Wen Chang RdTaipei Taiwan 111 an
Citazione:
K.G. Shyu et al., "Angiotensin II receptor antagonist blocks the expression of connexin43 induced by cyclical mechanical stretch in cultured neonatal rat cardiac myocytes", J MOL CEL C, 33(4), 2001, pp. 691-698

Abstract

Mechanical forces have profound effects on cardiomyocytes. To test whetherangiotensin IT is a potential mediator of stretch-induced effects on gap junctions, we used the angiotensin II (AT1) receptor antagonist, losartan, to investigate the cyclical stretch-induced expression of connexin43 (Cx43),the major cardiac muscle gap junction channel protein. Cultured neonatal rat cardiomyocytes grown on a flexible membrane base were stretched by vacuum to 20% of maximum elongation, at 60 cycles/min. The levels of Cx43 protein began to increase as early as 2 h after stretch was applied, reached a maximum of six-ford over the control by 24 h and remained at this level another 24 h (i.e. up to 45 h after stretch was applied). These increases of Cx43 protein at 24 h were largely (73%) and completely (100%) attenuated (P<0.001) by the addition (30 min before stretch) of 10 nM and 100 nM losartan, respectively. Similarly, the Cx43 mRNA levels in stretched cardiomyocytes rose 89% (P<0.01) above control (non-stretched cells) mRNA levels, This increase also was blocked by losartan, Cyclical stretch increased (and losartandecreased) the immunohistochemical labeling of Cx43 and significantly increased release of angiotensin II into the culture media from 7.5 +/-0.6 ng/ml to 23.8 +/-1.0ng/ml (P<0.01) after a Ih stretch. These findings indicate that cyclical mechanical stretch augments angiotensin II production and Cx43 gene expression in cultured cardiomyocytes, partially through mediation of the ATI receptors, and suggests interaction between the cardiomyocyte local rennin-angiotensin system and Cx43 in response to stretch, <(c)> 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:14:38