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Titolo:
alpha-Tocopherol decreases CD36 expression in human monocyte-derived macrophages
Autore:
Devaraj, S; Hugou, I; Jialal, I;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Pathol, Div Clin Biochem & Human Metab, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 iochem & Human Metab, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Dept Internal Med, Ctr Human Nutr, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Med, Ctr Human Nutr, Dallas, TX 75390 USA
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 4, volume: 42, anno: 2001,
pagine: 521 - 527
SICI:
0022-2275(200104)42:4<521:ADCEIH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; FOAM CELL-FORMATION; HUMAN-ENDOTHELIAL-CELLS; VITAMIN-E CONSUMPTION; B SCAVENGER RECEPTOR; OXIDIZED LDL; GLYCOPROTEIN-IV; PPAR-GAMMA; ATHEROSCLEROSIS; SUPPLEMENTATION;
Keywords:
atherosclerosis; scavenger receptor; LDL; antioxidant; vitamin E;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Jialal, I Univ Texas, SW Med Ctr, Dept Pathol, Div Clin Biochem & Human Metab, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 uman Metab, Dallas, TX 75390 USA
Citazione:
S. Devaraj et al., "alpha-Tocopherol decreases CD36 expression in human monocyte-derived macrophages", J LIPID RES, 42(4), 2001, pp. 521-527

Abstract

Cholesterol-laden macrophages are the hallmark of atherogenesis, The classB scavenger receptor, CD36, binds oxidized low density lipoprotein (OxLDL), is found in atherosclerotic lesions, and is upregulated by OxLDL, We tested the effects of or-tocopherol (AT) enrichment of human monocyte-derived macrophages on CD36 expression and cholesteryl ester accumulation. Monocytesisolated from normal volunteers were cultured into macrophages, Macrophages were enriched overnight with various doses of AT (25, 50, and 100 muM). LDL from normal volunteers was oxidized or acetylated (AcLDL) and incubated with macrophages for 48 h at a concentration of 50 or 100 mug/ml. CD36 expression was assessed by flow cytometry, Quantitative analysis of scavenger receptor class A (SR-A) activity was performed with 1,1'-dioctadecyl-3,3,3',3'-tetramethylin docarbocyanide perchlorate (DiI)-labeled LDL, CD36 expression was maximal after 8-10 days of culture, AT (greater than or equal to 50muM) significantly decreased CD36 expression upregulated by OxLDL and AcLDL (P < 0.01), Other antioxidants (<beta>- or gamma -tocopherol) or protein kinase C inhibitors failed to decrease CD36 expression, Concomitantly, DiI-AcLDL and DiI-OxLDL uptake was significantly decreased after AT treatment (P < 0.001), Cholesteryl ester accumulation was significantly decreased after AT enrichment (AcLDL + AT, 77% inhibition; OxLDL + AT, 42% inhibition). In conclusion, AT decreases both CD36 and SR-A expression and cholesteryl ester accumulation in human macrophages. This provides additional scientific support for the antiatherogenic properties of AT.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:30:30