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Titolo:
Induction of soluble antitumoral mediators by synthetic analogues of bacterial lipoprotein in bone marrow-derived macrophages from LPS-responder and -nonresponder mice
Autore:
Pfannes, SDC; Muller, B; Korner, S; Bessler, WG; Hoffmann, P;
Indirizzi:
Inst Mol Med & Zellforsch, AG Tumorimmunol & Vakzine, D-79104 Freiburg, Germany Inst Mol Med & Zellforsch Freiburg Germany D-79104 104 Freiburg, Germany Univ Freiburg, Fak Med, AG Biophys & Strahlenbiol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 enbiol, D-79104 Freiburg, Germany Jomol Pharma GmbH, Regensburg, Germany Jomol Pharma GmbH Regensburg Germany l Pharma GmbH, Regensburg, Germany
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 4, volume: 69, anno: 2001,
pagine: 590 - 597
SICI:
0741-5400(200104)69:4<590:IOSAMB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; NITRIC-OXIDE SYNTHASE; TUMOR CYTO-TOXICITY; PERITONEAL-MACROPHAGES; ACTIVATED MACROPHAGES; BORRELIA-BURGDORFERI; LIPOPEPTIDE ANALOGS; MURINE MACROPHAGES; MOUSE MACROPHAGES; L-ARGININE;
Keywords:
nitric oxide; oxidative burst; tumor necrosis factor-alpha; lipopeptide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Pfannes, SDC Inst Mol Med & Zellforsch, AG Tumorimmunol & Vakzine, Stefan Meier Str 8, D-79104 Freiburg, Germany Inst Mol Med & Zellforsch Stefan Meier Str 8 Freiburg Germany D-79104
Citazione:
S.D.C. Pfannes et al., "Induction of soluble antitumoral mediators by synthetic analogues of bacterial lipoprotein in bone marrow-derived macrophages from LPS-responder and -nonresponder mice", J LEUK BIOL, 69(4), 2001, pp. 590-597

Abstract

Macrophage-dependent antitumoral activity is partly mediated by soluble factors including cytokines, reactive-oxygen intermediates (ROIs), and reactive-nitrogen intermediates (RNIs), Activation of macrophages for tumor cytotoxicity can be achieved with various bacterial compounds, such as lipopolysaccharides (LPSs), muramyl-dipeptides, and lipopeptides. We studied the production and release of oxygen radicals, nitric oxide, and tumor necrosis factor alpha (TNF-alpha) by bone marrow-derived macrophages (BMDMs) of different mouse inbred strains after they were stimulated with the lipopeptide P3CSK4, a water-soluble synthetic analogue of the lipidated N terminus of bacterial lipoprotein. The lipopeptide was able to induce a strong, long lasting release of oxygen radicals in BALB/c mouse macrophages, Furthermore, it induced nitric oxide release from BMDMs of several mouse strains (BALB/c, C57BI/6, C57BI/10ScSn, Sv129, NMRI, and LPS-nonresponder C57BI/10ScCr), Stimulation with P3CSK4 also resulted in comparable production of TNF-alpha in LPS-responder and nonresponder BMDMs from C57BI/10ScSn mice and C57BI/10ScCr mice, respectively. All three antitumoral mediators reached functional levels or concentrations as shown by the strong cytostatic/cytotoxic activityof lipopeptide-activated macrophages for the cell lines Abelson 8-1, M12.5/P815, and L929, which are sensitive to ROIs, nitric oxide, and TNF-alpha, respectively. We found that synthetic lipopeptides can induce the secretionof effective levels of soluble tumor-cytotoxic/cytostatic mediators in BMDMs of LPS-responsive and, of particular interest, also of LPS-unresponsive mice. This result could indicate that the highly effective bacterial-macrophage activators P3CSK4 and LPS use different receptors and/or different intracellular signal transduction pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:34:33