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Titolo:
UVB-irradiated dendritic cells are impaired in their APC function and tolerize primed Th1 cells but not naive CD4+T cells
Autore:
Denfeld, RW; Hara, H; Tesmann, JP; Martin, S; Simon, JC;
Indirizzi:
Univ Freiburg, Dept Dermatol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 rmatol, D-79104 Freiburg, Germany Kitasato Univ, Sch Med, Dept Dermatol, Kanagawa, Japan Kitasato Univ Kanagawa Japan v, Sch Med, Dept Dermatol, Kanagawa, Japan
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 4, volume: 69, anno: 2001,
pagine: 548 - 554
SICI:
0741-5400(200104)69:4<548:UDCAII>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET-B RADIATION; HUMAN LANGERHANS CELLS; COSTIMULATORY MOLECULES; ANTIGEN; INDUCTION; LYMPHOCYTES; EXPRESSION; MECHANISM; INVITRO;
Keywords:
T lymphocyte; anergy; tolerance; costimulatory molecules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Denfeld, RW Univ Freiburg, Dept Dermatol, Hauptstr 7, D-79104 Freiburg, Germany Univ Freiburg Hauptstr 7 Freiburg Germany D-79104 rg, Germany
Citazione:
R.W. Denfeld et al., "UVB-irradiated dendritic cells are impaired in their APC function and tolerize primed Th1 cells but not naive CD4+T cells", J LEUK BIOL, 69(4), 2001, pp. 548-554

Abstract

We have shown that low-dose UVB radiation converts Langerhans cells (LC) front immunogenic to tolerogenic APC, Therefore, we questioned whether low-dose UVB irradiation of bone marrow-derived dendritic cells (DC) alters their APC function, thereby inducing tolerance in T cells. To address this issue, cocultures of DC; and naive, allogeneic T cells; naive, OVA-specific TCR-transgenic T cells from DO11.10 mice; or primed, antigen-specific T cells using the Th1 clone AE7 were analyzed. First, we found low-dose UVB-irradiated DC (UVB-DC) to dose-dependently (50-200 J/m(2)) inhibit T-cell proliferation of naive and primed T cells. In addition, supernatants harvested fromcocultures of UVB-DC and naive T cells showed markedly reduced levels of IL-2 and IFN-gamma and to a lesser degree of IL-4 and IL-IO, suggesting a preferential down-regulation of Th1 responses by UVB-DC, FAGS analysis of UVB-DC revealed no changes in surface expression of MHC, costimulatory, and adhesion molecules. To test tolerance induction, allo- or antigen-specific T cells isolated from cocultures with unirradiated DC and UVB-DC were restimulated with unirradiated DC or IL-2, It is interesting that UVB-DC induced antigen-specific tolerance in the Th1 clone AE?, In contrast, UVB-DC induceda partial inhibition of allogeneic T-cell proliferation but no tolerance with similar unresponsiveness to restimulation with IL-2 and unirradiated DCirrespective of their haplotype, Similar observations were made when naive, TCR-transgenic T cells from DO11.10 mice were used. In conclusion, UVB-DCare impaired in their APC function and tolerize the primed antigen-specific Th1 clone AE7 but not naive allo- or OVA-specific T cells.

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Documento generato il 04/04/20 alle ore 12:27:11