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Titolo:
Histone acetylation determines the developmentally regulated accessibilityfor T cell receptor gamma gene recombination
Autore:
Agata, Y; Katakai, T; Ye, SK; Sugai, M; Gonda, H; Honjo, T; Ikuta, K; Shimizu, A;
Indirizzi:
Kyoto Univ, Dept Mol Biol & Genet, Kyoto 6068507, Japan Kyoto Univ KyotoJapan 6068507 pt Mol Biol & Genet, Kyoto 6068507, Japan Kyoto Univ, Grad Sch Med, Dept Med Chem, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Med, Dept Med Chem, Kyoto 6068501, Japan
Titolo Testata:
JOURNAL OF EXPERIMENTAL MEDICINE
fascicolo: 7, volume: 193, anno: 2001,
pagine: 873 - 879
SICI:
0022-1007(20010402)193:7<873:HADTDR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
V(D)J RECOMBINATION; NUCLEOSOME STRUCTURE; CHROMATIN STRUCTURE; REARRANGEMENT; EXPRESSION; CLEAVAGE; LOCUS; DNA; TRANSCRIPTION; DIVERSITY;
Keywords:
V(D)J recombination; germline transcript; ligation-mediated PCR; chromatin immunoprecipitation; histone deacetylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Agata, Y Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 a Jolla, CA 92093 USA
Citazione:
Y. Agata et al., "Histone acetylation determines the developmentally regulated accessibilityfor T cell receptor gamma gene recombination", J EXP MED, 193(7), 2001, pp. 873-879

Abstract

Variable/diversity/joining (V[D]J) recombination of the T cell receptor (TCR) and immunoglobulin (Ig) genes is regulated by chromatin accessibility of the target locus to the recombinase in a lineage- and stage-specific manner. Histone acetylation has recently been proposed as a molecular mechanismunderlying the accessibility control. Here, we investigate the role for histone acetylation in the developmentally regulated rearrangements of the mouse TCR-gamma gene, wherein predominant rearrangement is switched from V gamma3 to V gamma2 gene during the fetal to adult thymocyte development. Our results indicate that histone acetylation correlates with accessibility, ashistone acetylation at the fetal-type V gamma3 gene in accord with germline transcription is relatively high in fetal thymocytes, but specifically becomes low in adult thymocytes within the entirely hyperacetylated locus. Furthermore, inhibition of histone deacetylation during the development of adult bone marrow-derived thymocytes by a specific histone deacetylase inhibitor, trichostatin A, leads to elevated histone acetylation, germline transcription, cleavage, and rearrangement of the V gamma3 gene. These data demonstrate that histone acetylation functionally determines the chromatin accessibility for V(D)J recombination in vivo and that an epigenetic modification of chromatin plays a direct role in executing a developmental switch in cell fate determination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:19:03