Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Chlamydial serology: Comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens
Autore:
Bas, S; Muzzin, P; Ninet, B; Bornand, JE; Scieux, C; Vischer, TL;
Indirizzi:
Univ Hosp, Dept Internal Med, Div Rheumatol, CH-1211 Geneva 14, Switzerland Univ Hosp Geneva Switzerland 14 heumatol, CH-1211 Geneva 14, Switzerland Univ Hosp, Div Infect Dis, CH-1211 Geneva, Switzerland Univ Hosp Geneva Switzerland CH-1211 ct Dis, CH-1211 Geneva, Switzerland Univ Hosp, Virol Lab, Dept Internal Med, CH-1211 Geneva 14, Switzerland Univ Hosp Geneva Switzerland 14 rnal Med, CH-1211 Geneva 14, Switzerland Geneva Med Sch, Dept Med Biochem, CH-1211 Geneva 14, Switzerland Geneva Med Sch Geneva Switzerland 14 hem, CH-1211 Geneva 14, Switzerland Hop St Louis, Bacteriol Lab, F-75475 Paris, France Hop St Louis Paris France F-75475 , Bacteriol Lab, F-75475 Paris, France
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 4, volume: 39, anno: 2001,
pagine: 1368 - 1377
SICI:
0095-1137(200104)39:4<1368:CSCDVO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MIP-LIKE PROTEIN; HUMORAL IMMUNE-RESPONSE; HEAT-SHOCK PROTEINS; LYME-DISEASE; TRACHOMATIS INFECTION; MONOCLONAL-ANTIBODIES; REACTIVE ARTHRITIS; PNEUMONIAE; PLASMID; HSP60;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bas, S Univ Hosp, Dept Internal Med, Div Rheumatol, CH-1211 Geneva 14, Switzerland Univ Hosp Geneva Switzerland 14 l, CH-1211 Geneva 14, Switzerland
Citazione:
S. Bas et al., "Chlamydial serology: Comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens", J CLIN MICR, 39(4), 2001, pp. 1368-1377

Abstract

To improve the reliability of the serodiagnosis of Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, anddifferent immunoassays using synthetic peptides derived front species-specific epitopes in variable domain IV of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipopolysaccharide) were evaluated, Because cross-reactions between chlamydial species have been reported, the microimmunofluorescence tests were also performed with Chlamydia pneumoniae and Chlamydia psittaci used as antigens, and C, pneumoniae-specific antibodies were also determined by immunoassays, Since the presence of antimicrobial antibodies must be interpreted in light of their prevalence inthe general population, responses obtained with serum samples from patients with well-defined infection (i.e., with positive urethral or endocervicalC, trachomatis DNA amplification) were compared to those obtained with samples from healthy blood donors, The best sensitivity (86%) with a specificity of 81% was obtained for immunoblotting results, when the number of individuals with greater than or equal to 10 immunoglobulin G (Igc) and/or greater than or equal to2 IgM responses to the different C, trachomatis antigenswas considered. A 13-kDa antigen was recognized by most of the samples (86% for IgG) front patients with acute urogenital infection but rarely (3%) by those from healthy blood donors (P < 0.0001), The sensitivity and specificity results obtained for serum antibodies to peptides or recombinant antigens were slightly lower than those results obtained for the number of responses to whole C, trachomatis antigens, which were 76 and 77%, respectively,when IgG responses to both recombinant hsp60 and pgp3 were considered.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:41:55