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Titolo:
Development of a high-performance liquid chromatographic method for the quantification of chlorpyrifos, pyridostigmine bromide, N,N-diethyl-m-toluamide and their metabolites in rat plasma and urine
Autore:
Abu-Qare, AW; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 533 - 538
SICI:
1387-2273(20010425)754:2<533:DOAHLC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT REPELLENT N,N-DIETHYL-M-TOLUAMIDE; SOLID-PHASE EXTRACTION; GULF-WAR SYNDROME; N,N-DIETHYL-META-TOLUAMIDE DEET; DERMAL ABSORPTION; PHARMACOKINETICS; EXPOSURE; QUANTITATION; EXCRETION;
Keywords:
chlorpyrifos; pyridostigmine bromide; N,N-diethyl-m-toluamide; combined exposure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27710USA Duke Univ POB 3813 Durham NC USA 27710 Durham, NC 27710USA
Citazione:
A.W. Abu-Qare e M.B. Abou-Donia, "Development of a high-performance liquid chromatographic method for the quantification of chlorpyrifos, pyridostigmine bromide, N,N-diethyl-m-toluamide and their metabolites in rat plasma and urine", J CHROMAT B, 754(2), 2001, pp. 533-538

Abstract

A method was developed for the separation and quantification of the insecticide chlorpyrifos (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphorothioate), its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphate) and TCP (3,5,6-trichloro-2-pyridinol), the anti-nerve agent drug pyridostigmine bromide (PB; 3-dimethylaminocarbonyloxy-N-methyl pyridinium bromide), its metabolite N-methyl-3-hydroxypyridinium bromide, the insect repellent DEET (N,N-diethyl-m-toluamide), and its metabolites m-toluamide and In-toluic acid in rat plasma and urine. The method is based onusing solid-phase extraction and high-performance liquid chromatography (HPLC) with reversed-phase C-18 column, and gradient UV detection ranging between 210 and 280 nm. The compounds were separated using a gradient of 1-85%acetonitrile in water (pH 3.20) at a how-rate ranging between 1 and 1.7 ml/min over a period of 15 min. The retention times ranged from 5.4 to 13.2 min. The limits of detection ranged between 20 and 150 ng/ml, while the Emits of quantitation were between 150 and 200 ng/ml. Average percentage recovery of five spilled plasma samples was 80.2+/-7.9, 74.9+/-8.5, 81.7+/-6.9, 73.1+/-7.8, 74.3+/-8.3, 80.8+/-6.6, 81.6+/-7.3 and 81.4+/-6.5, and from urine 79.4+/-6.9, 77.8+/-8.4, 83.3+/-6.6, 72.8+/-9.0, 76.3+/-7.7, 83.4+/-7.9, 81.6+/-7.9 and 81.8+/-6.8 for chlorpyrifos, chlorpyrifos-oxon, TCP, pyridostigmine bromide, N-methyl-3-hydroxypyridinium bromide, DEET, m-toluamide andm-toluic acid, respectively. The relationship between peak areas and concentration was linear over a range between 200 and 2000 ng/ml. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:34:05