Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Determination of growth hormone-releasing hexapeptide by reversed-phase high-performance liquid chromatography with electrochemical detection
Autore:
Choi, SJ; Lee, HY; Kim, SB; Kim, JH; Lee, SS; Yoo, SD; Lee, KC; Lee, HS;
Indirizzi:
Wonkwang Univ, Coll Pharm, Bioanal Lab, Iksan 570549, South Korea WonkwangUniv Iksan South Korea 570549 al Lab, Iksan 570549, South Korea Seoul Natl Univ, Sch Agr Biotechnol, Suwon 441744, South Korea Seoul Natl Univ Suwon South Korea 441744 hnol, Suwon 441744, South Korea Sung Kyun Kwan Univ, Coll Pharm, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 , Suwon 440746, South Korea
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 461 - 466
SICI:
1387-2273(20010425)754:2<461:DOGHHB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMON-CALCITONIN; PEPTIDE; SECRETAGOGUES; DEGRADATION;
Keywords:
growth-hormone-releasing peptide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, HS Wonkwang Univ, Coll Pharm, Bioanal Lab, Iksan 570549, South Korea Wonkwang Univ Iksan South Korea 570549 Iksan 570549, South Korea
Citazione:
S.J. Choi et al., "Determination of growth hormone-releasing hexapeptide by reversed-phase high-performance liquid chromatography with electrochemical detection", J CHROMAT B, 754(2), 2001, pp. 461-466

Abstract

A novel HPLC method with electrochemical detection is described for the determination of a growth-hormone-releasing hexapeptide (GHRP-6). HPLC conditions, such as the column, mobile phase, and oxidation potential, were optimized for sensitivity and selectivity of analysis. GHRP-6 was separated on areversed-phase CN column with 37% acetonitrile in 100 mM phosphate buffer (pH 7.0) as the mobile phase. The optimum electrochemical oxidation signal was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode dueto two electroactive tryptophans and a histidine residue. Solid-phase extraction using octadecyl cartridges was optimized for sample cleanup of GHRP-6 from serum samples and the method was successfully applied over the concentration range of 5 to 100 ng/ml of analyte. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:52:00