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Titolo:
Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids
Autore:
Seppanen-Laakso, T; Laakso, I; Vanhanen, H; Kiviranta, K; Lehtimaki, T; Hiltunen, R;
Indirizzi:
Univ Helsinki, Dept Pharm, Div Pharmacognosy, Bioctr 2, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 2, FIN-00014 Helsinki, Finland Univ Helsinki, Dept Med, Div Internal Med, Helsinki, Finland Univ Helsinki Helsinki Finland Med, Div Internal Med, Helsinki, Finland Univ Helsinki, Skin & Allergy Hosp, Helsinki, Finland Univ Helsinki Helsinki Finland , Skin & Allergy Hosp, Helsinki, Finland Univ Tampere, Sch Med, Dept Clin Chem, FIN-33101 Tampere, Finland Univ Tampere Tampere Finland FIN-33101 Chem, FIN-33101 Tampere, Finland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 437 - 445
SICI:
1387-2273(20010425)754:2<437:MHPLCD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT-SCATTERING DETECTION; SERUM-CHOLESTEROL; NEUTRAL LIPIDS; HEART-DISEASE; SEPARATION; QUANTIFICATION; IDENTIFICATION; ULTRAVIOLET; REPLACEMENT; MARGARINE;
Keywords:
evaporative light-scattering detection; cholesteryl esters; triacylglycerols; free cholesterol; phosphatidylcholine; phospholipid fatty acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Seppanen-Laakso, T Univ Helsinki, Dept Pharm, Div Pharmacognosy, Bioctr 2,POB 56, FIN-00014 Helsinki, Finland Univ Helsinki POB 56 Helsinki FinlandFIN-00014 inland
Citazione:
T. Seppanen-Laakso et al., "Major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids", J CHROMAT B, 754(2), 2001, pp. 437-445

Abstract

An HPLC method with evaporative light-scattering detection (ELSD) was optimized and validated for the simultaneous quantitation of cholesteryl esters(CEs), triacylglycerols (TGs), free cholesterol (FC) and phosphatidylcholine (PC) in human plasma. The separation of CEs from TGs, the most variable plasma lipid class, was improved by speeding up the gradient steps and by increasing the re-equilibration time between runs. The calibrations were made at levels of 0.14-14 mug lipid/injection. The intra- and inter-day precision values of the method ranged between 1.9 and 4.5 and 2.3-7.2% (RSD, n=6), respectively, including determinations at two concentration levels. In comparison to other lipid classes, quantitation of PC proved to be equally repeatable despite its lowest detector response. The relative recoveries varied from 97.0 to 110.3%, showing good accuracy of the method. The methodological variation of the lipid classes covered 0.6-3.1% of their total variation in the study population (n=48). The CE/FC ratio showed an even closer relationship with phospholipid linoleic acid (18:2n-6; r=0.65, P<0.001) than with serum cholesterol levels, while eicosapentaenoic acid (20:5n-3) was significantly associated with PC (r=0.41, P<0.01). The CE/FC ratio increased (P<0.01) during soyabean oil substitution and the level of PC increased (P<0.01) during cold-pressed rapeseed oil substitution. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 22/01/20 alle ore 06:48:59