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Titolo:
Affinity purification of novel bispecific antibodies recognising carcinoembryonic antigen and doxorubicin
Autore:
Ford, CHJ; Osborne, PO; Mathew, A; Rego, BG;
Indirizzi:
Kuwait Univ, Fac Med, Dept Surg, Safat 13110, Kuwait Kuwait Univ Safat Kuwait 13110 , Fac Med, Dept Surg, Safat 13110, Kuwait Mem Univ Newfoundland, Fac Med, Dept Surg, St Johns, NF, Canada Mem Univ Newfoundland St Johns NF Canada Dept Surg, St Johns, NF, Canada
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 427 - 435
SICI:
1387-2273(20010425)754:2<427:APONBA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL LYMPHOMA; MONOCLONAL-ANTIBODIES; TUMOR-CELLS; T-CELLS; CHROMATOGRAPHY; IMMUNOTHERAPY; COMBINATION; SAPORIN; CANCER;
Keywords:
bispecific antibodies; doxorubicin; carcinoembryonic antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Ford, CHJ Kuwait Univ, Fac Med, Dept Surg, POB 24923, Safat 13110, Kuwait Kuwait Univ POB 24923 Safat Kuwait 13110 3, Safat 13110, Kuwait
Citazione:
C.H.J. Ford et al., "Affinity purification of novel bispecific antibodies recognising carcinoembryonic antigen and doxorubicin", J CHROMAT B, 754(2), 2001, pp. 427-435

Abstract

We have developed a method which combines Protein A affinity chromatography and HPLC analytical and semipreparative hydroxyapatite affinity chromatography to purify bispecific antibodies (BsMabs) from hybrid-hybridomas secreting antibodies recognising carcinoembryonic antigen (CEA) and the chemotherapeutic drug doxorubicin (Dox). Elution of the HPLC hydroxyapatite columnswith a 60-360 mM phosphate buffer gradient was found to give better separation than elution with a 60-180 mM phosphate buffer gradient. Careful monitoring of HPLC fractions by enzyme linked immunosorbent assays for anti-CEA,anti-Dox and dual anti-CEA/anti-Dox activity, and pooling of fractions on the basis of these results, enabled the purification of novel BsMabs for use in in vitro and preclinical in vivo experiments. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:37:48