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Titolo:
Novel liquid chromatographic-tandem mass spectrometric methods using silica columns and aqueous-organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids
Autore:
Naidong, W; Shou, W; Chen, YL; Jiang, XY;
Indirizzi:
Covance Labs Inc, Madison, WI 53704 USA Covance Labs Inc Madison WI USA 53704 nce Labs Inc, Madison, WI 53704 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 387 - 399
SICI:
1387-2273(20010425)754:2<387:NLCMSM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMMONIUM NITRATE ELUENT; UV-ABSORBING COMPOUNDS; HUMAN PLASMA; RETENTION REPRODUCIBILITY; ELECTROSPRAY-IONIZATION; RAPID-DETERMINATION; BASIC COMPOUNDS; HUMAN URINE; SERUM; IDENTIFICATION;
Keywords:
silica columns; nicotine; cotinine; albuterol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Naidong, W Covance Labs Inc, 3301 Kinsman Blvd, Madison, WI 53704 USA Covance Labs Inc 3301 Kinsman Blvd Madison WI USA 53704 704 USA
Citazione:
W. Naidong et al., "Novel liquid chromatographic-tandem mass spectrometric methods using silica columns and aqueous-organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids", J CHROMAT B, 754(2), 2001, pp. 387-399

Abstract

Use of silica stationary phase and aqueous-organic mobile phases could significantly enhance LC-MS-MS method sensitivity. The LC conditions were compatible with MS detection. Analytes with basic functional groups were elutedwith acidic mobile phases and detected by MS in the positive ion mode. Analytes with acid functional groups were eluted with mobile phases at neutralpH and detected by MS in the negative ion mode. Analytes poorly retained on reversed-phase columns showed good retention on silica columns. Compared with reversed-phase LC-MS-MS, 5-8-fold sensitivity increases were observed for basic polar ionic compounds when using silica columns and aqueous-organic mobile phase. Up to a 20-fold sensitivity increase was observed for acidic polar ionic compounds. Silica columns and aqueous-organic mobile phases were used for assaying nicotine, cotinine, and albuterol in biological fluids. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:29:21