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Titolo:
High-performance liquid chromatographic determination of cefepime in humanplasma and in urine and dialysis fluid using a column-switching technique
Autore:
Cherti, N; Kinowski, JM; Lefrant, JY; Bressolle, F;
Indirizzi:
Univ Montpellier 1, Clin Pharmacokinet Lab, Montpellier, France Univ Montpellier 1 Montpellier France acokinet Lab, Montpellier, France Caremeau Hosp, Serv Pharm, Pharmacokinet Lab, Nimes, France Caremeau HospNimes France Serv Pharm, Pharmacokinet Lab, Nimes, France Fac Pharm Montpellier, Federat Anesthesiol Intens Care & Emergency, F-34060 Montpellier 2, France Fac Pharm Montpellier Montpellier France 2 F-34060 Montpellier 2, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 377 - 386
SICI:
1387-2273(20010425)754:2<377:HLCDOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHARMACOKINETICS; CEPHALOSPORIN; ANTIBIOTICS; VALIDATION; BMY-28142; CEFPIROME; SERUM; ASSAY;
Keywords:
cefepime;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Bressolle, F Fac Pharm Montpellier, Lab Pharmacocinet Clin, F-34060 Montpellier 2, France Fac Pharm Montpellier Montpellier France 2 ellier 2, France
Citazione:
N. Cherti et al., "High-performance liquid chromatographic determination of cefepime in humanplasma and in urine and dialysis fluid using a column-switching technique", J CHROMAT B, 754(2), 2001, pp. 377-386

Abstract

A high-performance liquid chromatographic method with UV absorbance was developed for the analysis of cefepime in human plasma and urine, and in dialysis fluid. Detection was performed at 280 nm. The assay procedure for cefepime in plasma involves the addition of an internal standard (cefpirome) followed by treatment of the samples with trichloracetic acid, acetonitrile and dichloromethane. To quantify cefepime in diluted urine (1:20) and in thedialysis fluid, samples spiked with the internal standard (cefpirome) wereanalysed using a column-switching technique. The HPLC column, Nucleosil C-18, was equilibrated with an eluent mixture composed of acetonitrile-ammonium acetate (pH 4). Linear detector responses were observed for the calibration curve standards in the range 0.5 to 100 mug/ml, which spans what is currently thought to be the clinically relevant range for cefepime concentrations in body fluids. The limit of quantification was 0.5 mug/ml in the threematrices. Extraction recoveries proved to be more than 84%. Precision, expressed as %RSD, was in the range 1.5 to 9%. Accuracy ranged from 93 to 105%. This method was used to follow the time course of the concentration of cefepime in plasma, urine and dialysate outlet samples after a 10-min infusion period of 2 g of this drug in patients with acute renal failure undergoing hemodiafiltration. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 21:41:37