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Titolo:
Improved method for the measurement of large neutral amino acids in biological matrices
Autore:
Bongiovanni, R; Yamamoto, BK; Jaskiw, GE;
Indirizzi:
Vet Affairs Med Ctr, Brecksville, OH 44141 USA Vet Affairs Med Ctr Brecksville OH USA 44141 r, Brecksville, OH 44141 USA Case Western Reserve Univ, Dept Psychiat, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 369 - 376
SICI:
1387-2273(20010425)754:2<369:IMFTMO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHIC DETERMINATION; CATECHOLAMINE SYNTHESIS; ORTHO-PHTHALALDEHYDE; PICOMOLE RANGE; RAT-BRAIN; PLASMA; DERIVATIZATION; TYROSINE; NEUROTRANSMITTERS; DOPAMINE;
Keywords:
derivatization, LC; amino acids, neutral;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bongiovanni, R Vet Affairs Med Ctr, GMBH,10000 Brecksville Rd, Brecksville, OH 44141 USA Vet Affairs Med Ctr GMBH,10000 Brecksville Rd Brecksville OHUSA 44141
Citazione:
R. Bongiovanni et al., "Improved method for the measurement of large neutral amino acids in biological matrices", J CHROMAT B, 754(2), 2001, pp. 369-376

Abstract

A high-performance liquid chromatographic method for measuring neutral amino acids in rat sera, brain tissues, and perfusates was developed by using omicron -phthalaldehyde sulfite as a pre-column derivatization reagent. With the present method, it was possible to separate the neutral amino acids within a single run in 25 min, while the acidic amino acids were eluted nearor at the solvent front. The recovery was above 88.8% with a relative standard deviation (RSD) below 4.2%. The within- and between-day assay reproducibility for the determination of rat serum amino acids showed RSDs below 1.35 and 7.61%, respectively. In the present study, the neutral amino acids were assayed with high sensitivity, accuracy and good reproducibility in a relatively short time and on a small sample size. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 05/04/20 alle ore 01:56:12