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Titolo:
Determination of isoprostaglandin F-2 alpha type III in human urine by gaschromatography-electronic impact mass spectrometry. Comparison with enzymeimmunoassay
Autore:
Bessard, J; Cracowski, JL; Stanke-Labesque, F; Bessard, G;
Indirizzi:
Grenoble Univ Hosp, Pharmacol Lab, F-38043 Grenoble 09, France Grenoble Univ Hosp Grenoble France 09 l Lab, F-38043 Grenoble 09, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 333 - 343
SICI:
1387-2273(20010425)754:2<333:DOIFAT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; PEROXIDATION IN-VIVO; LIPID-PEROXIDATION; 8-ISO-PROSTAGLANDIN F2-ALPHA; OXIDATIVE STRESS; 8-EPI-PROSTAGLANDIN F2-ALPHA; DEPENDENT FORMATION; F-2-ISOPROSTANES; PROSTAGLANDINS;
Keywords:
isoprostaglandin F-2 alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Bessard, J Grenoble Univ Hosp, Pharmacol Lab, BP 217, F-38043 Grenoble 09,France Grenoble Univ Hosp BP 217 Grenoble France 09 enoble 09, France
Citazione:
J. Bessard et al., "Determination of isoprostaglandin F-2 alpha type III in human urine by gaschromatography-electronic impact mass spectrometry. Comparison with enzymeimmunoassay", J CHROMAT B, 754(2), 2001, pp. 333-343

Abstract

F-2-Isoprostanes are stable lipid peroxidation products of arachidonic acid, the quantification of which provides an index of oxidative stress in vivo. We describe a method for analysing isoprostaglandin F-2 alpha type III (19-F-2 alpha-IsoP) in biological fluids. The method involves solid-phase extraction on octadecyl endcapped and aminopropyl cartridges. After conversion to trimethylsilyl ester trimethylsilyl ether derivatives, isoprostaglandin F-2 alpha type III is analysed by mass spectrometry, operated in electronic impact selected ion monitoring mode. We have compared enzyme immunoassay(EIA; Cayman, Ann Arbor, MI, USA) to this method with 30 human urine aliquots following the same extraction procedure in order to determine the agreement between both methods. Isoprostaglandin F-2 alpha type III concentrations determined with gas chromatography-mass spectrometry (GC-MS) did not agree with those determined with EIA. Our results suggest that GC-MS and EIA do not measure the same compounds. As a consequence, comparison of clinical results using GC-MS and EIA should be avoided. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 30/03/20 alle ore 10:15:07