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Titolo:
Simultaneous determination of Ziagen and its phosphorylated metabolites byion-pairing high-performance liquid chromatography-tandem mass spectrometry
Autore:
Fung, EN; Cai, ZW; Burnette, TC; Sinhababu, AK;
Indirizzi:
Glaxo Wellcome Inc, Bioanal & Drug Metab Div, Res Triangle Pk, NC 27709 USA Glaxo Wellcome Inc Res Triangle Pk NC USA 27709 Triangle Pk, NC 27709 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 754, anno: 2001,
pagine: 285 - 295
SICI:
1387-2273(20010425)754:2<285:SDOZAI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVERSED-PHASE; BIOLOGICAL SAMPLES; CYCLIC-NUCLEOTIDES; OLIGONUCLEOTIDES; PLASMA; TRIPHOSPHATE; NUCLEOBASES; SEPARATIONS; COMBINATION; ZIDOVUDINE;
Keywords:
Ziagen; carbovir phosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Cai, ZW Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Kowloon, Hong Kong, Peoples R China Hong Kong Baptist Univ Kowloon Tong Kowloon Hong Kong Peoples R China
Citazione:
E.N. Fung et al., "Simultaneous determination of Ziagen and its phosphorylated metabolites byion-pairing high-performance liquid chromatography-tandem mass spectrometry", J CHROMAT B, 754(2), 2001, pp. 285-295

Abstract

An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N',N'-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found tobe better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 muM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:04:44