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Titolo:
Astrocytes as a predominant cellular site of Tc-99m-HMPAO retention
Autore:
Zerarka, S; Pellerin, L; Slosman, D; Magistretti, PJ;
Indirizzi:
Univ Lausanne, Inst Physiol, CH-1005 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1005 CH-1005 Lausanne, Switzerland Univ Lausanne, Serv Neurol, CH-1005 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1005 CH-1005 Lausanne, Switzerland Cantonal Univ Hosp, Dept Nucl Engn, Geneva, Switzerland Cantonal Univ Hosp Geneva Switzerland pt Nucl Engn, Geneva, Switzerland
Titolo Testata:
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
fascicolo: 4, volume: 21, anno: 2001,
pagine: 456 - 468
SICI:
0271-678X(200104)21:4<456:AAAPCS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HM-PAO STEREOISOMERS; CEREBRAL BLOOD-FLOW; PROPYLENEAMINE OXIME; GLUTATHIONE CONTENT; BRAIN SPECT; CULTURES; NEURONS; AGENT; ACID; TECHNETIUM-99M-MESO-HMPAO;
Keywords:
SPECT imaging; brain energy metabolism; astrocytes; neurons; glutathion; cerebral blood flow;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Magistretti, PJ Univ Lausanne, Inst Physiol, 7 Rue Bugnon, CH-1005 Lausanne, Switzerland Univ Lausanne 7 Rue Bugnon Lausanne Switzerland CH-1005 d
Citazione:
S. Zerarka et al., "Astrocytes as a predominant cellular site of Tc-99m-HMPAO retention", J CEREBR B, 21(4), 2001, pp. 456-468

Abstract

Technetium-99m-d,1-hexamethylpropylene amine oxime (Tc-99m-HMPAO) retention in the brain monitored by single photon emission computed tomography (SPECT) is currently used as a marker of cerebral blood flow. The purported mechanism by which Tc-99m-HMPAO accumulates in the brain is through its intracellular conversion from a lipophilic form to more hydrophilic derivatives within the brain parenchyma. The issue of the contribution of different celltypes on Tc-99m-HMPAO retention was investigated in vitro by studying the accumulation of Tc-99m-HMPAO in primary cultures of mouse cortical astrocytes and neurons. Results show that Tc-99m-HMPAO retention predominates in astrocytes over neurons by a factor of similar to2.5 (0.26 +/- 0.05 vs. 0.095+/- 0.042 fmol/mg protein after 120 minutes, respectively). Diethyl maleate (60 mu mol/L), ethacrynic acid (1 mmol/L) and buthionine sulfoximine (1 mmol/L), 3 agents which significantly reduced glutathione levels also decreased Tc-99m-HMPAO retention in both astrocytes (29%, 3%, and 46% of control,respectively) and neurons (69%, 11% and 63% of control). Decrease did not always correlate with glutathione levels, however, which suggests that other factors could be involved. The possibility that cell energy status determines Tc-99m-HMPAO retention was also assessed. Agents that activate (glutamate, azide) or inhibit (cytochalasin B) glucose utilization in astrocytes, as measured by the H-3-2-deoxyglucose method, were without effect on Tc-99m-HMPAO retention. In conclusion, the data presented indicate that astrocytes may constitute a prominent site of Tc-99m-HMPAO retention and most likelycontribute significantly to the SPECT signal. In addition. the data also suggest that specific alterations in glial cell metabolism could explain flow-independent changes in Tc-99m-HMPAO retention in the brain as observed bySPECT in some pathologies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 14:01:47