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Titolo:
Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous
Autore:
Sen, A; Lea-Currie, YR; Sujkowska, D; Franklin, DM; Wilkison, WO; Halvorsen, YDC; Gimble, JM;
Indirizzi:
ZenBio Inc, Res Triangle Pk, NC 27709 USA ZenBio Inc Res Triangle Pk NC USA 27709 nc, Res Triangle Pk, NC 27709 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 2, volume: 81, anno: 2001,
pagine: 312 - 319
SICI:
0730-2312(2001)81:2<312:APOHAD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-BINDING PROTEIN; FOS SIGNALING PATHWAYS; AP2 GENE-EXPRESSION; MESSENGER-RNA; OBESE GENE; PPAR-GAMMA; DIFFERENTIATION; ENHANCER; TISSUE; DNA;
Keywords:
adipose; stromal cells; heterogeneous; branched DNA; aP2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sen, A ZenBio Inc, POB 12503,3200 Chapel Hill Nelson Blvd,Suite 104, Res TrianglePk, NC 27709 USA ZenBio Inc POB 12503,3200 Chapel Hill Nelson Blvd,Suite 104 Res Triangle Pk NC USA 27709
Citazione:
A. Sen et al., "Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous", J CELL BIOC, 81(2), 2001, pp. 312-319

Abstract

The current study was done to assess if heterogeneity existed in the degree of adipogenesis in stromal cells (preadipocytes) from multiple donors. Inaddition to conventional lipid-based methods, we have employed a novel signal amplification technology, known as branched DNA, to monitor expression of an adipocyte specific gene product aP2. The fatty acid binding protein aP2 increases during adipocyte differentiation and is induced by thiazolidinediones and other peroxisome proliferator activated receptor gamma ligands. The current work examined the adipogenic induction of aP2 mRNA levels in human adipose tissue stromal cells derived from 12 patients (mean age +/- SEM, 38.9 +/- 3.1) with mild to moderate obesity (mean body mass index +/- SEM, 27.8 +/- 2.4). Based on branched DNA technology, a rapid and sensitive measure of specific RNAs the relative aP2 level in adipocytes increased by 679 +/- 93-fold (mean +/- SEM, n = 12) compared to preadipocytes. Normalization of the aP2 mRNA levels to the housekeeping gene, glyceraldehyde phosphate dehydrogenase, did not significantly alter the fold induction in a subset of 4 patients (803.6 +/- 197.5 vs 1118.5 +/- 308.1). Independent adipocyte differentiation markers were compared between adipocytes and preadipocytes in parallel studies. Leptin secretion increased by up to three-orders of magnitude while measurements of neutral lipid accumulation by Oil Red O andNile Red staining increased by 8.5-fold and 8.3-fold, respectively. These results indicate that preadipocytes isolated from multiple donors displayedvarying degrees of differentiation in response to an optimal adipogenic stimulus in vitro. This work also demonstrates that branched DNA measurement of aP2 is a rapid and sensitive measure of adipogenesis in human stromal cells. The linear range of this assay extends up to three-orders of magnitudeand correlates directly with independent measures of cellular differentiation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 13:04:11