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Titolo:
Paranemin and the organization of desmin filament networks
Autore:
Schweitzer, SC; Klymkowsky, MW; Bellin, RM; Robson, RM; Capetanaki, Y; Evans, RM;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Pathol, Denver, CO 80262 USA Univ Colorado, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309Univ Colorado, Boulder, CO 80309 USA Iowa State Univ Sci & Technol, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA Iowa State Univ Sci & Technol Ames IA USA 50011 Biol, Ames, IA 50011 USA Iowa State Univ Sci & Technol, Dept Anim Sci, Ames, IA 50011 USA Iowa State Univ Sci & Technol Ames IA USA 50011 m Sci, Ames, IA 50011 USA Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Cell Biol, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 6, volume: 114, anno: 2001,
pagine: 1079 - 1089
SICI:
0021-9533(200103)114:6<1079:PATOOD>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICK MYOTUBES INVIVO; ALPHA-B-CRYSTALLIN; KNOCK-OUT MICE; PROTEIN SYNEMIN; MOLECULAR CHARACTERISTICS; REGULATED EXPRESSION; SKELETAL MYOPATHY; MISSENSE MUTATION; EPITHELIAL-CELLS; MITOTIC CELLS;
Keywords:
desmin; vimentin; paranemin; synemin; nestin; intermediate filament;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Evans, RM Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Pathol, Denver, CO 80262 USA
Citazione:
S.C. Schweitzer et al., "Paranemin and the organization of desmin filament networks", J CELL SCI, 114(6), 2001, pp. 1079-1089

Abstract

De novo expression of vimentin, GFAP or peripherin leads to the assembly of an extended intermediate filament network in intermediate filament-free SW13/cl.2 cells, Desmin, in contrast, does not form extended filament networks: in either SW13/cl.2 or intermediate filament-free mouse fibroblasts, Rather, desmin formed short thickened filamentous structures and prominent spot-like cytoplasmic aggregates that were composed of densely packed 9-11 nmdiameter filaments, Analysis of stably transfected cell lines indicates that the inability of desmin to form extended networks is not due to a difference in the level of transgene expression. Nestin, paranemin and synemin are large intermediate filament proteins that coassemble with desmin in muscle cells. Although each of these large intermediate filament proteins colocalized with desmin when coexpressed in SW-13 cells, expression of paranemin,bat not synemin or nestin, led to the formation of an extended desmin network. A similar rescue of desmin network organization was observed when desmin was coexpressed with vimentin, which coassembles with desmin, or with keratins, which formed a distinct filament network. These studies demonstratethat desmin filaments differ in their organizational properties from the other vimentin-like intermediate filament proteins and appear to depend uponcoassembly with paranemin, at least when they are expressed in non-muscle cells, in order to form an extended filament network.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/05/20 alle ore 02:10:44