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Titolo:
Identification of a basolateral sorting signal for the m(3) muscarinic acetylcholine receptor in Madin-Darby canine kidney cells
Autore:
Nadler, LS; Kumar, G; Nathanson, NM;
Indirizzi:
Univ Washington, Sch Med, Dept Pharmacol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pharmacol, Seattle, WA 98195 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 13, volume: 276, anno: 2001,
pagine: 10539 - 10547
SICI:
0021-9258(20010330)276:13<10539:IOABSS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLARIZED EPITHELIAL-CELLS; PROTEIN-COUPLED RECEPTORS; GROWTH-FACTOR RECEPTOR; MDCK CELLS; STRUCTURAL REQUIREMENTS; CYTOPLASMIC TAIL; HIPPOCAMPAL-NEURONS; MOLECULAR-CLONING; XENOPUS OOCYTES; ACINAR-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Nathanson, NM Univ Washington, Sch Med, Dept Pharmacol, POB 357750, Seattle, WA 98195 USA Univ Washington POB 357750 Seattle WA USA 98195 WA 98195 USA
Citazione:
L.S. Nadler et al., "Identification of a basolateral sorting signal for the m(3) muscarinic acetylcholine receptor in Madin-Darby canine kidney cells", J BIOL CHEM, 276(13), 2001, pp. 10539-10547

Abstract

Muscarinic acetylcholine receptors (mAChRs) can be differentially localized in polarized cells. To identify potential sorting signals that mediate mAChR targeting, we examined the sorting of mAChRs in Madin-Darby canine kidney cells, a widely used model system. Expression of FLAG-tagged mAChRs in polarized Madin-Darby canine kidney cells demonstrated that the M-2 subtype is sorted apically, whereas M-3 is targeted basolaterally, Expression of M-2/M-3 receptor chimeras revealed that a al-residue sequence, Ser(271)-Ser(291), from the M-3 third intracellular loop contains a basolateral sorting signal. Substitution of sequences containing the M-3 sorting signal into thehomologous regions of M-2 was sufficient to confer basolateral localization to this apical receptor. Sequences containing the M-3 sorting signal alsoconferred basolateral targeting to M-2 when added to either the third intracellular loop or the C-terminal cytoplasmic tail. Furthermore, addition ofa sequence containing the M-3 basolateral sorting signal to the cytoplasmic tail of the interleukin-2 receptor alpha -chain caused significant basolateral targeting of this heterologous apical protein. The results indicate that the M-3 basolateral sorting signal is dominant over apical signals in M-2 and acts in a position-independent manner. The M-3 sorting signal represents a novel basolateral targeting motif for G protein-coupled receptors.

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Documento generato il 30/11/20 alle ore 20:07:49