Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Surface characteristics of Bacillus cereus and its adhesion to stainless steel
Autore:
Peng, JS; Tsai, WC; Chou, CC;
Indirizzi:
Natl Taiwan Univ, Grad Inst Food Sci & Technol, Taipei, Taiwan Natl TaiwanUniv Taipei Taiwan Inst Food Sci & Technol, Taipei, Taiwan Food Ind Res & Dev Inst, Hsinchu 300, Taiwan Food Ind Res & Dev Inst Hsinchu Taiwan 300 Dev Inst, Hsinchu 300, Taiwan
Titolo Testata:
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
fascicolo: 1-2, volume: 65, anno: 2001,
pagine: 105 - 111
SICI:
0168-1605(20010411)65:1-2<105:SCOBCA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
LISTERIA-MONOCYTOGENES; BACTERIAL ATTACHMENT; MILK COMPONENTS; SOLID-SURFACES; HYDROPHOBICITY; SPORES;
Keywords:
Bacillus cereus; surface characteristics; adhesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Chou, CC Natl Taiwan Univ, Grad Inst Food Sci & Technol, 59 Lane 144,Keelung Rd,Sec4, Taipei, Taiwan Natl Taiwan Univ 59 Lane 144,Keelung Rd,Sec 4 Taipei Taiwan wan
Citazione:
J.S. Peng et al., "Surface characteristics of Bacillus cereus and its adhesion to stainless steel", INT J F MIC, 65(1-2), 2001, pp. 105-111

Abstract

The ability of a Bacillus cereus strain, isolated from spoiled milk, to adhere to the surface of stainless steel chips was evaluated during its growth in diluted tryptic soy broth (DTSB). The number of cells that adhered to the surface increased markedly as the culture reached the end of the log phase and entered stationary phase, and continued to increase with Further incubation. The surface properties of cells from the log, stationary, and late stationary phases were measured by hydrophobic interaction chromatography(HIC) and electrostatic interaction chromatography (ESIC). It was found that surface hydrophobicity of B. cereus vegetative cells from the late stationary phase was the highest followed by those from the stationary phase andthe log phase cultures. While the vegetative cells prepared from stationary phase and log phase cultures, respectively, had the highest and the lowest surface charges. Adhesion of B. cereus vegetative cells to stainless steel was positively correlated with the cell surface hydrophobicity (R = 0.979). Surface hydrophobicity and surface positive charge noted on the spores harvested from diluted tryptic soy agar (DTSA) and Mn2+-tryptone glucose extract agar were higher than those harvested from the sucrose or lactose-added DTSA. A wide variation in the surface charge values was noted on the surface of various spores prepared from cultures grown on the four different media tested, while their ability to adhere to stainless steel chips in phosphate buffered saline (PBS) showed no significant difference (p > 0.05). Similarly, the number of spores or vegetative cells adhering to stainless steel suspended in PBS, milk or diluted milk (1000 X) did not differ significantly (p > 0.05). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:50:40