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Titolo:
Oxygen modulates the release of urokinase and plasminogen activator inhibitor-1 by retinal pigment epithelial cells
Autore:
Erichsen, JT; Jarvis-Evans, J; Khaliq, A; Boulton, M;
Indirizzi:
Cardiff Univ, Dept Optometry & Vis Sci, Cardiff CF10 3NB, S Glam, Wales Cardiff Univ Cardiff S Glam Wales CF10 3NB ardiff CF10 3NB, S Glam, Wales Univ Manchester, Dept Ophthalmol, Manchester, Lancs, England Univ Manchester Manchester Lancs England mol, Manchester, Lancs, England Univ Manchester, Sch Biol Sci, Manchester, Lancs, England Univ ManchesterManchester Lancs England Sci, Manchester, Lancs, England
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 3, volume: 33, anno: 2001,
pagine: 237 - 247
SICI:
1357-2725(200103)33:3<237:OMTROU>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; MASSIVE PERIRETINAL PROLIFERATION; VASCULAR ENDOTHELIAL-CELLS; FACTOR MESSENGER-RNA; BASIC FIBROBLAST; IN-VITRO; GENE-EXPRESSION; MATRIX METALLOPROTEINASES; EXTRACELLULAR PROTEOLYSIS; PERICELLULAR PROTEOLYSIS;
Keywords:
oxygen; plasminogen activator; plasminogen activator inhibitor-1; retinal pigment epithelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Boulton, M Cardiff Univ, Dept Optometry & Vis Sci, Cardiff CF10 3NB, S Glam, Wales Cardiff Univ Cardiff S Glam Wales CF10 3NB 3NB, S Glam, Wales
Citazione:
J.T. Erichsen et al., "Oxygen modulates the release of urokinase and plasminogen activator inhibitor-1 by retinal pigment epithelial cells", INT J BIO C, 33(3), 2001, pp. 237-247

Abstract

The aims of this study were to examine the effect of oxygen, in the: presence or absence of exogenous growth factors, on the release of plasminogen activators and plasminogen activator inhibitor-1 by cultured human retinal pigment epithelial cells. Antigen and activity levels of urokinase, tissue plasminogen activator and plasminogen activator inhibitor were measured in conditioned media after cells were exposed to three different oxygen environments: hypoxia. normoxia and hyperoxia. Overall proteolylic balance was determined by zymography. The effects of exogenous basic fibroblast growth factor and transforming growth factor-beta were also examined, it was found that retinal pigment epithelial cells released urokinase, tissue plasminogen activator and plasminogen activator inhibitor in measurable quantities. After 48 h, urokinase levels were highest at normoxia, reaching 7.2ng/10(6) cells (+/- 2.0 SEM), whereas plasminogen activator inhibitor 1 levels were highest at hyperoxia, reaching 67.5ng/10(6) cells (+/- 3.7 SEM). Tissue plasminogen activator levels were minimal ( < 0.5ng/10(6) cells) and unaffected by both oxygen and growth factors. Overall proteolytic activity was also greatest at normoxia. Fibroblast growth Factor stimulated urokinase production dose-dependently, but plasminogen activator inhibitor only minimally. Transforming growth factor-beta stimulated plasminogen activator inhibitor production dose-dependently but urokinase only at higher concentrations. Theseresults suggest that both oxygen tension and growth factors may interact to modulate the proteolytic properties of the human retinal pigment epithelium. <(c)> 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:00:24