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Titolo:
Evidence for a role of ganglioside GM(1) in antigen presentation: binding enhances presentation of Escherichia coli enterotoxin B subunit (EtxB) to CD4(+) T cells
Autore:
Nashar, TO; Betteridge, ZE; Mitchell, RN;
Indirizzi:
Univ Bristol, Sch Med Sci, Dept Pathol & Microbiol, Bristol BS8 1TD, Avon,England Univ Bristol Bristol Avon England BS8 1TD , Bristol BS8 1TD, Avon,England Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 pt Pathol, Boston, MA 02115 USA Harvard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 vard Univ, Sch Med, Boston, MA 02115 USA
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 4, volume: 13, anno: 2001,
pagine: 541 - 551
SICI:
0953-8178(200104)13:4<541:EFAROG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-LABILE ENTEROTOXIN; CHOLERA-TOXIN; CLASS-II; ANTIBODY-RESPONSES; RECEPTOR-BINDING; DENDRITIC CELLS; EXOGENOUS ANTIGENS; PROTEIN ANTIGENS; ORAL TOLERANCE; LIPID RAFTS;
Keywords:
antigen binding; antigen presentation; CtxB; enterotoxin; EtxB; GM(1);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Nashar, TO Univ Bristol, Sch Med Sci, Dept Pathol & Microbiol, Bristol BS81TD, Avon,England Univ Bristol Bristol Avon England BS8 1TD S8 1TD, Avon,England
Citazione:
T.O. Nashar et al., "Evidence for a role of ganglioside GM(1) in antigen presentation: binding enhances presentation of Escherichia coli enterotoxin B subunit (EtxB) to CD4(+) T cells", INT IMMUNOL, 13(4), 2001, pp. 541-551

Abstract

Successful antigen presentation by antigen-presenting cells is governed bya number of factors including the efficiency of antigen capture by cell-surface receptors, targeting to compartments of antigen processing, surface expression of MHC II-peptide complexes and presence of costimulatory signals. Ganglioside GM(1) is an important component of membrane glycosphingolipids, and has been implicated in cell differentiation, apoptosis and signal transduction pathways. Using the a subunit of Escherichia coll enterotoxin (EtxB), a potent immunogen that binds GM1 with high affinity, and a non-binding mutant of EtxB, EtxB((G33D)), we demonstrate that GM(1) is intimately involved in several aspects of antigen presentation. Thus, GM(1)-mediated presentation of EtxB by a cells and CD11c(+) dendritic cells (DC) significantly enhanced the proliferation and cytokine expression of EtxB-specific CD4(+) T cells. Investigation regarding potential mechanisms revealed that EtxB binding directly augments the expression of MHC class H on B cells, and fractionation of a cells demonstrated that EtxB binding to GM(1) results in rapid internalization and targeting to class II-rich compartments. GM(1)-mediated uptake of antigens and access to class II compartments in B cells can be exploited to significantly enhance the presentation of ovalbumin-conjugated to EtxB. These results demonstrate that GM(1) can play an important role in antigen presentation via the MHC II pathway.

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Documento generato il 25/01/20 alle ore 16:10:03