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Titolo:
From transcription to cell surface expression, the induction of MHC class III-A alpha by interferon-gamma in macrophages is regulated at different levels
Autore:
Cullell-Young, M; Barrachina, M; Lopez-Lopez, C; Gonalons, E; Lloberas, J; Soler, C; Celada, A;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain Univ Barcelona, Fdn August Pi & Sunyer, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 Sunyer, E-08028 Barcelona, Spain
Titolo Testata:
IMMUNOGENETICS
fascicolo: 2, volume: 53, anno: 2001,
pagine: 136 - 144
SICI:
0093-7711(200103)53:2<136:FTTCSE>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HISTOCOMPATIBILITY COMPLEX; INVARIANT CHAIN; GENE-EXPRESSION; MICE LACKING; IFN-GAMMA; TRANSACTIVATOR CIITA; TUMORICIDAL ACTIVITY; B-CELLS; MOLECULES;
Keywords:
transcription; translation; protein synthesis; major histocompatibility complex; class II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Celada, A Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, Av Diagonal645, E-08028 Barcelona, Spain Univ Barcelona Av Diagonal 645 Barcelona Spain E-08028 a, Spain
Citazione:
M. Cullell-Young et al., "From transcription to cell surface expression, the induction of MHC class III-A alpha by interferon-gamma in macrophages is regulated at different levels", IMMUNOGENET, 53(2), 2001, pp. 136-144

Abstract

Using mouse bone marrow-derived macrophages we determined the role of interferon (IFN)-gamma at the different steps in expression of the I-A alpha chain of MHC class II molecules, from transcription to the cell surface. Levels of transcription, RNA, and protein were low in cells not stimulated withIFN-gamma. Treat ment with IFN-gamma for 24 or 48 h induced an increase inmRNA levels (7- and 12-fold) that did not correlate with the increase in transcription (2.5- and 2.7-fold). The half-life of mRNA was not modified byIFN-gamma. These data suggest a block at the level of translation. In fact, IFN-gamma increased ribosome loading, which confirms regulation at the translational level. Treatment with IFN-gamma increased protein synthesis (6-fold after 48 h) and level of expression at the cell surface (3- and 9-foldafter 24 and 48 h, respectively). Interestingly, treatment with IFN-gamma also increased the I-A alpha protein half-life from 2 to 6-7 h. This is thefirst attempt to determine qualitatively and quantitatively the regulationof an inducible gene at all the putative levels of control. The data indicate that IFN-gamma plays a critical role in MHC class II protein expressionin macrophages through the regulation of different steps, from transcription to surface expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:19:37