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Titolo:
Towards a new generation of vaccines: the cytokine IL-12 as an adjuvant toenhance cellular immune responses to pathogens during prime-booster vaccination regimens
Autore:
Gherardi, MM; Ramirez, JC; Esteban, M;
Indirizzi:
CSIC, Ctr Nacl Biotecnol, Dept Mol & Cellular Biol, Madrid, Spain CSIC Madrid Spain cl Biotecnol, Dept Mol & Cellular Biol, Madrid, Spain CONICET, Ctr Bot & Phamracol Studies, RA-1033 Buenos Aires, DF, Argentina CONICET Buenos Aires DF Argentina RA-1033 033 Buenos Aires, DF, Argentina
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 2, volume: 16, anno: 2001,
pagine: 655 - 667
SICI:
0213-3911(200104)16:2<655:TANGOV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING-FACTOR; CYTOTOXIC T-LYMPHOCYTES; NITRIC-OXIDE; VIRUS ANKARA; PROTECTIVE EFFICACY; DNA VACCINES; IN-VIVO; RECOMBINANT INTERLEUKIN-12; MUCOSAL IMMUNITY; INTERFERON-GAMMA;
Keywords:
immune modulation; cytokines; IL-12; vaccines; prime/boost; HIV; vaccinia virus recombinants;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Esteban, M CSIC, Ctr Nacl Biotecnol, Dept Mol & Cellular Biol, Campus UnivAutonoma, Madrid, Spain CSIC Campus Univ Autonoma Madrid Spain utonoma, Madrid, Spain
Citazione:
M.M. Gherardi et al., "Towards a new generation of vaccines: the cytokine IL-12 as an adjuvant toenhance cellular immune responses to pathogens during prime-booster vaccination regimens", HIST HISTOP, 16(2), 2001, pp. 655-667

Abstract

A main goal of the industrialized world is the development of effective vaccines to control infectious diseases with major health and socio-economic impact. Current understanding of the immune response triggered during infection with pathogens causing malaria, hepatitis C and AIDS emphasizes the importance of cytotoxic T lymphocytes (CTLs) in combating these infections. This has led to the development of new vaccination strategies, some of whichare in phase I/II clinical trials. Promising strategies of vaccination arebased on highly attenuated viral vectors, such as Vaccinia virus (VV) in combination with heterologous like vectors naked DNA, referred to as priming/booster vaccination. While these immunization schedules increased the production of specific CTLs, there is a need to further expand the CD8+T cell population to control an infection. Among molecules that play a significant role in the modulation of the CTL response is the cytokine IL-12. Immunoregulation by IL-12 is of central importance in cell-mediated immunity (CMI) against those pathogens and tumors that are controlled by cell-mediated mechanisms, supported by Th1 cells. The use of this cytokine in combination with highly immunogenic VV-derived vectors is a promising system for development of future vaccination schedules. In this review, we summarize recent data on the use of IL-12 in vaccination procedures, as well as undesired side-effects of the cytokine that can be overcome by accurate use of dose, routeand time-window administration of IL-12 encoding vectors. Results described here indicate that VV IL-12-mediated enhancement of the specific CMI response against a model antigen HIV-1 env was time- and dose-dependent and that the antigen and the cytokine could be expresed from two different rVVs modulating the doses of the vectors and allowing for enhancement of a specific CMI response. Moreover, the use of IL-12 during DNA prime/VV boost regimens enhanced the specific anti-HIV-1 env cellular response 20 times comparedto that generated after a single rVVenv inoculation. Variables such as: a)dose of the cytokine applied, b) time of its administration and c) routes of inoculation play a critical role in the final outcome of the response. The findings presented here can be extended to other antigens, suggesting that immunomodulatory cytokines can be useful in the development of the future vaccines against numerous infectious diseases and tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:11:34