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Titolo:
Process formation of podocytes: morphogenetic activity of microtubules andregulation by protein serine/threonine phosphatase PP2A
Autore:
Kobayashi, N; Reiser, J; Schwarz, K; Sakai, T; Kriz, W; Mundel, P;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Div Nephrol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Juntendo Univ, Sch Med, Dept Anat, Tokyo 113, Japan Juntendo Univ Tokyo Japan 113 Univ, Sch Med, Dept Anat, Tokyo 113, Japan Univ Heidelberg, Dept Anat & Cell Biol, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 115, anno: 2001,
pagine: 255 - 266
SICI:
0948-6143(200103)115:3<255:PFOPMA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PHEOCHROMOCYTOMA PC12 CELLS; OKADAIC ACID; NEURONAL DIFFERENTIATION; INTERMEDIATE FILAMENTS; TYROSINE PHOSPHATASES; SIGNAL-TRANSDUCTION; NEURITE OUTGROWTH; CALYCULIN-A; PHOSPHORYLATION;
Keywords:
podocyte (glomerular epithelial cell); cytoskeleton; microtubules; protein phosphorylation; protein phosphatase type 2A (PP2A);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Mundel, P Yeshiva Univ Albert Einstein Coll Med, Div Nephrol, 1300 Morris Pk Ave, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave Bronx NY USA 10461
Citazione:
N. Kobayashi et al., "Process formation of podocytes: morphogenetic activity of microtubules andregulation by protein serine/threonine phosphatase PP2A", HISTOCHEM C, 115(3), 2001, pp. 255-266

Abstract

Podocytes possess major processes containing microtubules (MTs) and intermediate filaments and foot processes containing actin filaments (AFs) as core cytoskeletal elements. Although the importance of these cytoskeletal elements for maintaining podocyte processes was previously shown, so far no data are available concerning the developmental regulation of podocyte processformation. A conditionally immortalized mouse podocyte cell line, which can be induced to develop processes similar to those found in vivo, was treated with various reagents to disrupt cytoskeletal elements or to inhibit protein phosphatases, MTs colocalized with vimentin intermediate filaments butnot with AFs. After AF disassembly, major processes were maintained, whereas after depolymerization of MTs, podocytes lost their processes, rounded up, and maintained only actin-based pe ripheral projections. Suppression of MT elongation by nanomolar vinblastine or inhibition of serine/threonine phosphatase PP2A with okadaic acid abolished process formation. PP2A was expressed in undifferentiated but not in differentiated podocytes. One- and two-dimensional western blot analyses revealed a dose-dependent increase in serine/threonine phosphorylation after okadaic acid treatment. Hence, morphogenetic activity of MTs induces podocyte process formation via serine/threonine protein dephosphorylation by PP2A. These results may open new avenues for understanding the signaling mechanism underlying podocyte cytoskeleton alterations during development and in glomerular diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 19:33:00