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Titolo:
Subcellular localization of Dp71 dystrophin isoforms in cultured hippocampal neurons and forebrain astrocytes
Autore:
Aleman, V; Osorio, B; Chavez, O; Rendon, A; Mornet, D; Martinez, D;
Indirizzi:
Inst Politecn Nacl, CINVESTAV, Dept Fisiol Biofis & Neurociencias, Mexico City 07000, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico 07000 ico City 07000, DF, Mexico Inst Bouisson Bertrand, INSERM, U128, Grp Muscles & Pathol, F-34196 Montpellier 5, France Inst Bouisson Bertrand Montpellier France 5 -34196 Montpellier 5, France INSERM, EMI 99 18, Lab Physiopathol Cellulaire & Mol Retine, F-67091 Strasbourg, France INSERM Strasbourg France F-67091 Mol Retine, F-67091 Strasbourg, France
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 115, anno: 2001,
pagine: 243 - 254
SICI:
0948-6143(200103)115:3<243:SLODDI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUCHENNE MUSCULAR-DYSTROPHY; PROTEIN-KINASE-C; ELECTRON-MICROSCOPIC LOCALIZATION; CENTRAL-NERVOUS-SYSTEM; NONMUSCLE TISSUES; NUCLEAR-ENVELOPE; RAT HIPPOCAMPUS; MAJOR PRODUCT; BREFELDIN-A; BRAIN;
Keywords:
Dp71 isoforms; dystrophin; hippocampal neurons; forebrain astrocytes; Duchenne muscular dystrophy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Aleman, V Inst Politecn Nacl, CINVESTAV, Dept Fisiol Biofis & Neurociencias, Apdo Postal 14-740, Mexico City 07000, DF, Mexico Inst Politecn Nacl Apdo Postal 14-740 Mexico City DF Mexico 07000
Citazione:
V. Aleman et al., "Subcellular localization of Dp71 dystrophin isoforms in cultured hippocampal neurons and forebrain astrocytes", HISTOCHEM C, 115(3), 2001, pp. 243-254

Abstract

It has been suggested that the absence or altered structure of Dp71, a C-terminal dystrophin gene encoded protein, is responsible for mental alterations observed in about 30% of Duchenne muscular dystrophy patients. Most of these patients have premature translational termination or point mutations at the C-terminal domain of this gene. In brain, Dp71 is the major protein product of the dystrophin gene. To determine the func tion of Dp71 isoformsin this organ, it is important to document their presence and intracellular localization in brain cells. Extracts from cultured hippocampal neurons and forebrain astrocytes and 5F3 and Dys 2 monoclonal antibodies were thus used for western blots. In these conditions, two Dp71 isoforms spliced or not at exon 78 were detected in both cells (Dp71f and Dp71d, respectively). By immunocytochemistry. we mapped Dp71f and Dp71d in the Golgi complex (GC) and in neuronal nuclei. Only Dp71d was found in cytoplasmic neurofilaments. In astrocytes, these isoforms were detected in the GC, These cell localization data suggest that these Dp71 isoforms may have different functions in the same cell or organelle, as well as in the two different cells analyzed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 21:07:48