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Titolo:
Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in lgf2 imprinting during pre-implantation mouse development
Autore:
Ohno, M; Aoki, N; Sasaki, H;
Indirizzi:
Natl Inst Genet, Dept Integrated Genet, Div Human Genet, Shizuoka 4118540,Japan Natl Inst Genet Shizuoka Japan 4118540 man Genet, Shizuoka 4118540,Japan Kyushu Univ, Inst Genet Informat, Div Dis Genes, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Div Dis Genes, Fukuoka 8128582, Japan Grad Univ Adv Studies, Dept Genet, Shizuoka 4118540, Japan Grad Univ Adv Studies Shizuoka Japan 4118540 et, Shizuoka 4118540, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 3, volume: 6, anno: 2001,
pagine: 249 - 259
SICI:
1356-9597(200103)6:3<249:ADONTB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IGF2 GENE; EXPRESSION; EMBRYOS; PREIMPLANTATION; METHYLATION; H19; ACTIVATION; MECHANISMS; CHROMATIN; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, H Natl Inst Genet, Dept Integrated Genet, Div Human Genet, Shizuoka 4118540,Japan Natl Inst Genet Shizuoka Japan 4118540 Shizuoka 4118540,Japan
Citazione:
M. Ohno et al., "Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in lgf2 imprinting during pre-implantation mouse development", GENES CELLS, 6(3), 2001, pp. 249-259

Abstract

Background: Genomic imprinting causes parental-origin-specific monoallelictranscription of a subset of mammalian genes in the embryo and adult. There is conflicting evidence, however, for the monoallelic transcription of some imprinted genes, such as Igf2, in pre-implantation embryos. Results: We have developed an allele-specific fluorescence in situ hybridization method which involves a pair of oligonucleotide probes designed to detect an intronic polymorphism. The method, called ASO-RNA-FISH, enabled usto distinguish allelic nascent Igf2 transcripts in the cell. nuclei of early mouse embryos, avoiding signals from the stored oocyte-specific transcripts, Igf2 transcription was first detectable in two-cell embryos, and biallelic transcription was predominant up to the morula stage. Then, the maternal allele became silenced during the blastocyst stage. When embryos were cultured in vitro, however, a strong bias to maternal transcription was observed up to the morula stage. Conclusion: ASO-RNA-FISH revealed that a transition of Igf2 from biallelicto monoallelic transcription occurs in the blastocyst stage. This developmental regulation was modified temporarily by in vitro culture, suggesting apossible link between altered imprinting and abnormalities of the foetusesexperienced in vitro culture, ASO-RNA-FISH is therefore a powerful technique for the study of allele-specific gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 06:21:30