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Titolo:
Genetic and physical mapping of two centromere-proximal regions of chromosome IV in Aspergillus nidulans
Autore:
Aleksenko, A; Nielsen, ML; Clutterbuck, AJ;
Indirizzi:
Tech Univ Denmark, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 echnol, DK-2800 Lyngby, Denmark Univ Glasgow, Inst Biomed & Life Sci, Glasgow G11 6NU, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G11 6NU ow G11 6NU, Lanark, Scotland
Titolo Testata:
FUNGAL GENETICS AND BIOLOGY
fascicolo: 1, volume: 32, anno: 2001,
pagine: 45 - 54
SICI:
1087-1845(200102)32:1<45:GAPMOT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROSPORA-CRASSA; DNA; CLONING; MUTANT; MAP;
Keywords:
AMA1; Aspergillus nidulans; centromere; chromosome walking; genetic map; physical map;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Aleksenko, A Tech Univ Denmark, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 00 Lyngby, Denmark
Citazione:
A. Aleksenko et al., "Genetic and physical mapping of two centromere-proximal regions of chromosome IV in Aspergillus nidulans", FUNGAL G B, 32(1), 2001, pp. 45-54

Abstract

The centromere-proximal portion of the chromosome was mapped physically using overlapping clones of a cosmid genomic library. Two contiguous segmentsof a physical map, based on restriction mapping of cosmid clones, were generated, together covering more than 0.4 Mb DNA. A reverse genetic mapping approach was used to establish a correlation between physical and genetic maps; i,e., marker genes were integrated into physically mapped segments and subsequently mapped by mitotic and meiotic recombination. The resulting data, together with additional classical genetic mapping, lead to a substantial revision of the genetic map of the chromosome, including the position of the centromere, Comparison of physical and genetic maps indicates that meiotic recombination is low in subcentromeric DNA, its frequency being reducedfrom 1 crossover per 0.8 Mb to approximately 1 crossover per 5 Mb per meiosis, The portion of the chromosome containing the functional centromere wasnot mapped because repeat-rich regions hindered further chromosome walking. The size of the missing segment was estimated to be between 70 and 400 kb. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:01:36