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Titolo:
Transport of L-carnitine in isolated cerebral cortex neurons
Autore:
Wawrzenczyk, A; Sacher, A; Mac, M; Nalecz, MJ; Nalecz, KA;
Indirizzi:
M Nencki Inst Expt Biol, Dept Mol & Cellular Neurobiol, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Warsaw Poland PL-02093 , PL-02093 Warsaw, Poland Tel Aviv Univ, Dept Biochem, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 iochem, IL-69978 Tel Aviv, Israel
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 7, volume: 268, anno: 2001,
pagine: 2091 - 2098
SICI:
0014-2956(200104)268:7<2091:TOLIIC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; BLOOD-BRAIN-BARRIER; NEUROBLASTOMA NB-2A CELLS; ORGANIC CATION TRANSPORTER; TISSUE DISTRIBUTION PATTERN; KINASE-C ACTIVITY; RAT-BRAIN; NEUROTRANSMITTER TRANSPORTERS; IN-VITRO; PHARMACOLOGICAL CHARACTERIZATION;
Keywords:
gamma-aminobutyric acid; carnitine; neurons; transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Nalecz, KA M Nencki Inst Expt Biol, Dept Mol & Cellular Neurobiol, PasteurSt 3, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Pasteur St 3 WarsawPoland PL-02093 nd
Citazione:
A. Wawrzenczyk et al., "Transport of L-carnitine in isolated cerebral cortex neurons", EUR J BIOCH, 268(7), 2001, pp. 2091-2098

Abstract

The accumulation of carnitine was measured in cerebral cortex neurons isolated from adult rat brain. This process was found to be lowered by 40% after preincubation with ouabain and with SH-group reagents (N-ethylmaleimide and mersalyl). The initial velocity of carnitine transport was found to be inhibited by LF-aminobutyrate (GABA) in a competitive way (K-i = 20.9 +/- 2.4 mM). However, of various inhibitors of GABA transporters, only nipecotic acid and very high concentrations of 1-[2-([(diphenylmethylene) amino] oxy)ethyl] -1,2,5,6-tetrahydro-3 -pyridine-carboxylic acid hydrochloride (NO-711) acid decreased carnitine accumulation while betaine, taurine and p-alanine had no effect. The GABA transporters expressed in Xenopus laevis oocytesdid not transport carnitine. Moreover, carnitine was not observed to diminish the accumulation of GABA in cerebral cortex neurons, which further excluded a possible involvement of the GABA transporter GAT1 in the process of carnitine accumulation, despite the expression of this protein in the cellsunder study. The absence of carnitine transporter OCTN2 in rat cerebral cortex neurons (K. A. Nalecz, D. Dymna, J. E. Mroczkowska, A. Broer, S. BroerM. J. Nalecz and R. Cecchelli, unpublished results), together with the insensitivity of carnitine accumulation towards betaines, implies that a noveltransporting protein is present in these cells.

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Documento generato il 03/07/20 alle ore 22:46:14